αB-Crystallin monoclonal antibody (1B6.1-3G4) | Enzo Life Sciences ADI-SPA-222-F product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

αB-Crystallin monoclonal antibody (1B6.1-3G4)

catalog :

ADI-SPA-222-F

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1B6.1-3G4

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Enzo Life Sciences product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 1	Nivon M, Fort L, Muller P, Richet E, Simon S, Guey B, et al. NF?B is a central regulator of protein quality control in response to protein aggregation stresses via autophagy modulation. Mol Biol Cell. 2016;27:1712-27 pubmed publisher
western blot; human; fig 1	Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, et al. A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis. PLoS Genet. 2015;11:e1005582 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig 5	Watanabe Y, Kazuki Y, Kazuki K, Ebiki M, Nakanishi M, Nakamura K, et al. Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis. Mol Ther Nucleic Acids. 2015;4:e253 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 4b	Malin D, Strekalova E, Petrovic V, Rajanala H, Sharma B, Ugolkov A, et al. ERK-regulated Î±B-crystallin induction by matrix detachment inhibits anoikis and promotes lung metastasis in vivo. Oncogene. 2015;34:5626-34 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Bellaye P, Burgy O, Colas J, Fabre A, Marchal Sommé J, Crestani B, et al. Antifibrotic role of ?B-crystallin inhibition in pleural and subpleural fibrosis. Am J Respir Cell Mol Biol. 2015;52:244-52 pubmed publisher
western blot; human; 1:1000	Frankenberg N, Lamb G, Overgaard K, Murphy R, Vissing K. Small heat shock proteins translocate to the cytoskeleton in human skeletal muscle following eccentric exercise independently of phosphorylation. J Appl Physiol (1985). 2014;116:1463-72 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:100 immunocytochemistry; rat; 1:200 western blot; rat	Tang S, Lv Y, Chen H, Adam A, Cheng Y, Hartung J, et al. Comparative analysis of ?B-crystallin expression in heat-stressed myocardial cells in vivo and in vitro. PLoS ONE. 2014;9:e86937 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200 western blot; human	Malin D, Strekalova E, Petrovic V, Deal A, Al Ahmad A, Adamo B, et al. ?B-crystallin: a novel regulator of breast cancer metastasis to the brain. Clin Cancer Res. 2014;20:56-67 pubmed publisher
	Judge L, Perez Bermejo J, Truong A, Ribeiro A, Yoo J, Jensen C, et al. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI Insight. 2017;2: pubmed publisher
	McLendon P, Davis G, Gulick J, Singh S, Xu N, Salomonis N, et al. An Unbiased High-Throughput Screen to Identify Novel Effectors That Impact on Cardiomyocyte Aggregate Levels. Circ Res. 2017;121:604-616 pubmed publisher
	Chandrasekaran M, Chun S. Expression of PR-protein genes and induction of defense-related enzymes by Bacillus subtilis CBR05 in tomato (Solanum lycopersicum) plants challenged with Erwinia carotovora subsp. carotovora. Biosci Biotechnol Biochem. 2016;80:2277-2283 pubmed publisher
	Toft D, Fuller M, Schipma M, Chen F, Cryns V, Layden B. ?B-crystallin and HspB2 deficiency is protective from diet-induced glucose intolerance. Genom Data. 2016;9:10-7 pubmed publisher
	Huang Y, Wang Z, Liu Y, Xiong H, Zhao Y, Wu L, et al. Î±B-Crystallin Interacts with Nav1.5 and Regulates Ubiquitination and Internalization of Cell Surface Nav1.5. J Biol Chem. 2016;291:11030-41 pubmed publisher
	Liu R, Li S, Garcia E, Glubrecht D, Poon H, Easaw J, et al. Association between cytoplasmic CRABP2, altered retinoic acid signaling, and poor prognosis in glioblastoma. Glia. 2016;64:963-76 pubmed publisher
	Quraishe S, Wyttenbach A, Matinyarare N, Perry V, Fern R, O CONNOR V. Selective and compartmentalized myelin expression of HspB5. Neuroscience. 2016;316:130-42 pubmed publisher
	Tang S, Chen H, Cheng Y, Nasir M, Kemper N, Bao E. Expression profiles of heat shock protein 27 and Î±B-crystallin and their effects on heat-stressed rat myocardial cells in vitro and in vivo. Mol Med Rep. 2016;13:1633-8 pubmed publisher
	Kim M, Lee H, Lee E. Renal tumor with alpha B crystallin expression. Int J Clin Exp Pathol. 2015;8:9383-9 pubmed
	Kim M, Lee H, Jun S, Lee E. Expression of alpha B crystallin and BCL2 in patients with infiltrating ductal carcinoma. Int J Clin Exp Pathol. 2015;8:8842-56 pubmed
	Xu G, Fromholt S, Ayers J, Brown H, Siemienski Z, Crosby K, et al. Substantially elevating the levels of Î±B-crystallin in spinal motor neurons of mutant SOD1 mice does not significantly delay paralysis or attenuate mutant protein aggregation. J Neurochem. 2015;133:452-64 pubmed publisher
	Moberg C, Bourlev V, Ilyasova N, Olovsson M. Levels of oestrogen receptor, progesterone receptor and ?B-crystallin in eutopic endometrium in relation to pregnancy in women with endometriosis. Hum Fertil (Camb). 2015;18:30-7 pubmed publisher
	Volkmann J, Reuning U, Rudelius M, Häfner N, Schuster T, Becker V Ros A, et al. High expression of crystallin ?B represents an independent molecular marker for unfavourable ovarian cancer patient outcome and impairs TRAIL- and cisplatin-induced apoptosis in human ovarian cancer cells. Int J Cancer. 2013;132:2820-32 pubmed publisher
	Pannabecker T. Loop of Henle interaction with interstitial nodal spaces in the renal inner medulla. Am J Physiol Renal Physiol. 2008;295:F1744-51 pubmed publisher
	Mu T, Ong D, Wang Y, Balch W, Yates J, Segatori L, et al. Chemical and biological approaches synergize to ameliorate protein-folding diseases. Cell. 2008;134:769-81 pubmed publisher
	Rao N, Saraswathy S, Wu G, Katselis G, Wawrousek E, Bhat S. Elevated retina-specific expression of the small heat shock protein, alphaA-crystallin, is associated with photoreceptor protection in experimental uveitis. Invest Ophthalmol Vis Sci. 2008;49:1161-71 pubmed publisher
	Kamradt M, Chen F, Cryns V. The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation. J Biol Chem. 2001;276:16059-63 pubmed

product information

Product ID :

ADI-SPA-222

Catalog Number :

ADI-SPA-222-F

Product Name :

αB-Crystallin monoclonal antibody (1B6.1-3G4)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

200 µg

list of Pubmed id :

26718604, 26464626, 25684139, 23225306, 18842821, 18775310, 18326745, 11274139

Clone :

1B6.1-3G4

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Chicken, Bovine

Background :

Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.

ALTnames :

CRYAB, HSPB5

Immunogen :

Bovine αB-crystallin.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~20kDa by Western blot.

Application Summary :

ICC, IF, IHC (PS), WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage