HO-1 monoclonal antibody (HO-1-2) | Enzo Life Sciences ADI-OSA-111-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HO-1 monoclonal antibody (HO-1-2)

catalog :

ADI-OSA-111-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HO-1-2

The same clone is also sold as:

Boster: M00253-1
Abcam: ab83214, ab69545

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 2	Belcher J, Chen C, Nguyen J, Zhang P, Abdulla F, Nguyen P, et al. Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate. Antioxid Redox Signal. 2017;26:748-762 pubmed publisher
western blot; mouse	Vercellotti G, Khan F, Nguyen J, Chen C, Bruzzone C, Bechtel H, et al. H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice. Front Pharmacol. 2014;5:79 pubmed publisher
	Zhao H, Kalish F, Wong R, Stevenson D. Infiltration of myeloid cells in the pregnant uterus is affected by heme oxygenase-1. J Leukoc Biol. 2017;101:217-226 pubmed publisher
	Rebolledo R, Van Erp A, Ottens P, Wiersema Buist J, Leuvenink H, Romanque P. Anti-Apoptotic Effects of 3,3',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats. PLoS ONE. 2015;10:e0138749 pubmed publisher
	Choi E, Park H, Sul O, Rajasekaran M, Yu R, Choi H. Carbon monoxide reverses adipose tissue inflammation and insulin resistance upon loss of ovarian function. Am J Physiol Endocrinol Metab. 2015;308:E621-30 pubmed publisher
	Viquez O, Lai B, Ahn J, Does M, Valentine H, Valentine W. N,N-diethyldithiocarbamate promotes oxidative stress prior to myelin structural changes and increases myelin copper content. Toxicol Appl Pharmacol. 2009;239:71-9 pubmed publisher
	Takagi T, Naito Y, Mizushima K, Nukigi Y, Okada H, Suzuki T, et al. Increased intestinal expression of heme oxygenase-1 and its localization in patients with ulcerative colitis. J Gastroenterol Hepatol. 2008;23 Suppl 2:S229-33 pubmed publisher
	Peng P, Ko M, Chen C, Juan S. Haem oxygenase-1 gene transfer protects retinal ganglion cells from ischaemia/reperfusion injury. Clin Sci (Lond). 2008;115:335-42 pubmed publisher
	Gueler F, Park J, Rong S, Kirsch T, Lindschau C, Zheng W, et al. Statins attenuate ischemia-reperfusion injury by inducing heme oxygenase-1 in infiltrating macrophages. Am J Pathol. 2007;170:1192-9 pubmed
	Whitekus M, Li N, Zhang M, Wang M, Horwitz M, Nelson S, et al. Thiol antioxidants inhibit the adjuvant effects of aerosolized diesel exhaust particles in a murine model for ovalbumin sensitization. J Immunol. 2002;168:2560-7 pubmed
	Terajima H, Enders G, Thiaener A, Hammer C, Kondo T, Thiery J, et al. Impact of hyperthermic preconditioning on postischemic hepatic microcirculatory disturbances in an isolated perfusion model of the rat liver. Hepatology. 2000;31:407-15 pubmed
	Panahian N, Yoshiura M, Maines M. Overexpression of heme oxygenase-1 is neuroprotective in a model of permanent middle cerebral artery occlusion in transgenic mice. J Neurochem. 1999;72:1187-203 pubmed
	Maines M, Polevoda B, Coban T, Johnson K, Stoliar S, Huang T, et al. Neuronal overexpression of heme oxygenase-1 correlates with an attenuated exploratory behavior and causes an increase in neuronal NADPH diaphorase staining. J Neurochem. 1998;70:2057-69 pubmed

product information

Product ID :

ADI-OSA-111

Catalog Number :

ADI-OSA-111-D

Product Name :

HO-1 monoclonal antibody (HO-1-2)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26437380, 19467251, 19120903, 18341478, 17392159, 11859152, 10655264, 10037492, 9572292

Image Legend 4 :

Immunohistochemistry analysis of frozen mouse spinal cord with HO-1, mAb (HO-1-2).

Clone :

HO-1-2

Isotype :

IgG2b

SPECIES REACTIVITY :

Human, Mouse, Rat, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog

Background :

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

ALTnames :

HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1

Immunogen :

Native rat HO-1.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~32kDa by Western blot.

Application Summary :

FC, IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage