product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HO-1 monoclonal antibody (HO-1-1)
catalog :
ADI-OSA-110-D
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HO-1-1

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, other
more info or order :
Enzo Life Sciences product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:2000; loading ...; fig 2b
  • western blot; human; 1:2000; loading ...; fig 7a
Pajares M, Jiménez Moreno N, García Yagüe A, Escoll M, De Ceballos M, Van Leuven F, et al. Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes. Autophagy. 2016;12:1902-1916 pubmed
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
Vodret S, Bortolussi G, Jašprová J, Vitek L, Muro A. Inflammatory signature of cerebellar neurodegeneration during neonatal hyperbilirubinemia in Ugt1 -/- mouse model. J Neuroinflammation. 2017;14:64 pubmed publisher
Lin Y, Jean Y, Lee H, Lin S, Pan C, Chen W, et al. Excavatolide B Attenuates Rheumatoid Arthritis through the Inhibition of Osteoclastogenesis. Mar Drugs. 2017;15: pubmed publisher
Starheim K, Holien T, Misund K, Johansson I, Baranowska K, Sponaas A, et al. Intracellular glutathione determines bortezomib cytotoxicity in multiple myeloma cells. Blood Cancer J. 2016;6:e446 pubmed publisher
Nishimoto S, Suzuki T, Koike S, Yuan B, Takagi N, Ogasawara Y. Nrf2 activation ameliorates cytotoxic effects of arsenic trioxide in acute promyelocytic leukemia cells through increased glutathione levels and arsenic efflux from cells. Toxicol Appl Pharmacol. 2016;305:161-168 pubmed publisher
Wang L, Yokoyama K, Lin C, Chen T, Hsiao H, Chiang P, et al. Knockout of ho-1 protects the striatum from ferrous iron-induced injury in a male-specific manner in mice. Sci Rep. 2016;6:26358 pubmed publisher
Rubio Navarro A, Carril M, Padro D, Guerrero Hue M, Tarin C, Samaniego R, et al. CD163-Macrophages Are Involved in Rhabdomyolysis-Induced Kidney Injury and May Be Detected by MRI with Targeted Gold-Coated Iron Oxide Nanoparticles. Theranostics. 2016;6:896-914 pubmed publisher
Deliyanti D, Lee J, Petratos S, Meyer C, Ward K, Wilkinson Berka J, et al. A potent Nrf2 activator, dh404, bolsters antioxidant capacity in glial cells and attenuates ischaemic retinopathy. Clin Sci (Lond). 2016;130:1375-87 pubmed publisher
Oh S, Hwang J, Lee Y, Choi S, Kim J, Kim J, et al. Isolation of basal membrane proteins from BeWo cells and their expression in placentas from fetal growth-restricted pregnancies. Placenta. 2016;39:24-32 pubmed publisher
Tibullo D, Barbagallo I, Giallongo C, Vanella L, Conticello C, Romano A, et al. Heme oxygenase-1 nuclear translocation regulates bortezomibinduced cytotoxicity and mediates genomic instability in myeloma cells. Oncotarget. 2016;7:28868-80 pubmed publisher
Wichmann H, Vocke F, Brinkhoff T, Simon M, Richter Landsberg C. Cytotoxic Effects of Tropodithietic Acid on Mammalian Clonal Cell Lines of Neuronal and Glial Origin. Mar Drugs. 2015;13:7113-23 pubmed publisher
Pettersen K, Monsen V, Hakvåg Pettersen C, Overland H, Pettersen G, Samdal H, et al. DHA-induced stress response in human colon cancer cells - Focus on oxidative stress and autophagy. Free Radic Biol Med. 2016;90:158-72 pubmed publisher
Harada S, Nakagawa T, Yokoe S, Edogawa S, Takeuchi T, Inoue T, et al. Autophagy Deficiency Diminishes Indomethacin-Induced Intestinal Epithelial Cell Damage through Activation of the ERK/Nrf2/HO-1 Pathway. J Pharmacol Exp Ther. 2015;355:353-61 pubmed publisher
Ishima Y, Narisoko T, Kragh Hansen U, Kotani S, Nakajima M, Otagiri M, et al. Nitration of indoxyl sulfate facilitates its cytotoxicity in human renal proximal tubular cells via expression of heme oxygenase-1. Biochem Biophys Res Commun. 2015;465:481-7 pubmed publisher
Chen H, Lu P, Chen B, Hsiao M, Ho W, Tseng C. Heme oxygenase-1 ameliorates kidney ischemia-reperfusion injury in mice through extracellular signal-regulated kinase 1/2-enhanced tubular epithelium proliferation. Biochim Biophys Acta. 2015;1852:2195-201 pubmed publisher
Nguyen Ho Bouldoires T, Clapéron A, Mergey M, Wendum D, Desbois Mouthon C, Tahraoui S, et al. Mitogen-activated protein kinase-activated protein kinase 2 mediates resistance to hydrogen peroxide-induced oxidative stress in human hepatobiliary cancer cells. Free Radic Biol Med. 2015;89:34-46 pubmed publisher
Lee S, Yi C, Heo R, Song D, Cho Y, Jeong Y, et al. Clarithromycin Attenuates Radiation-Induced Lung Injury in Mice. PLoS ONE. 2015;10:e0131671 pubmed publisher
Choi S, Paek H, Yu J. Oxidative stress by layered double hydroxide nanoparticles via an SFK-JNK and p38-NF-κB signaling pathway mediates induction of interleukin-6 and interleukin-8 in human lung epithelial cells. Int J Nanomedicine. 2015;10:3217-29 pubmed publisher
Jabs A, Oelze M, Mikhed Y, Stamm P, Kröller Schön S, Welschof P, et al. Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats. Vascul Pharmacol. 2015;71:181-91 pubmed publisher
Crowston J, Kong Y, Trounce I, Dang T, Fahy E, Bui B, et al. An acute intraocular pressure challenge to assess retinal ganglion cell injury and recovery in the mouse. Exp Eye Res. 2015;141:3-8 pubmed publisher
Kawashiri M, Nakanishi C, Tsubokawa T, Shimojima M, Yoshida S, Yoshimuta T, et al. Impact of Enhanced Production of Endogenous Heme Oxygenase-1 by Pitavastatin on Survival and Functional Activities of Bone Marrow-derived Mesenchymal Stem Cells. J Cardiovasc Pharmacol. 2015;65:601-6 pubmed publisher
Lawal A, Zhang M, Dittmar M, Lulla A, Araujo J. Heme oxygenase-1 protects endothelial cells from the toxicity of air pollutant chemicals. Toxicol Appl Pharmacol. 2015;284:281-291 pubmed publisher
Vogler M, Zieseniss A, Hesse A, Levent E, Tiburcy M, Heinze E, et al. Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult. Pflugers Arch. 2015;467:2141-9 pubmed publisher
Kang H, Yi Y, Hong Y, Kim H, Jang Y, Seong Y, et al. HER2 confers drug resistance of human breast cancer cells through activation of NRF2 by direct interaction. Sci Rep. 2014;4:7201 pubmed publisher
Gemici B, Tan R, Birsen I, İzgüt Uysal V. Gastroprotective effect of orexin-A and heme oxygenase system. J Surg Res. 2015;193:626-33 pubmed publisher
Lundvig D, Scharstuhl A, Cremers N, Pennings S, Te Paske J, van Rheden R, et al. Delayed cutaneous wound closure in HO-2 deficient mice despite normal HO-1 expression. J Cell Mol Med. 2014;18:2488-98 pubmed publisher
Li L, Yang N, Nin L, Zhao Z, Chen L, Yu J, et al. Chinese herbal medicine formula tao hong si wu decoction protects against cerebral ischemia-reperfusion injury via PI3K/Akt and the Nrf2 signaling pathway. J Nat Med. 2015;69:76-85 pubmed publisher
Yamashita Y, Ueyama T, Nishi T, Yamamoto Y, Kawakoshi A, Sunami S, et al. Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole. PLoS ONE. 2014;9:e97419 pubmed publisher
Gleixner A, Pulugulla S, Pant D, Posimo J, Crum T, Leak R. Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat. Cell Tissue Res. 2014;357:43-54 pubmed publisher
Kongpetch S, Kukongviriyapan V, Prawan A, Senggunprai L, Kukongviriyapan U, Buranrat B. Crucial role of heme oxygenase-1 on the sensitivity of cholangiocarcinoma cells to chemotherapeutic agents. PLoS ONE. 2012;7:e34994 pubmed publisher
Jenkins N, Liu T, Cassidy P, Leachman S, Boucher K, Goodson A, et al. The p16(INK4A) tumor suppressor regulates cellular oxidative stress. Oncogene. 2011;30:265-74 pubmed publisher
product information
Product ID :
ADI-OSA-110
Catalog Number :
ADI-OSA-110-D
Product Name :
HO-1 monoclonal antibody (HO-1-1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
26633426, 26585906, 26404472, 26277392, 26232688, 25869522, 25620054, 25467193, 25263508, 24723229
Clone :
HO-1-1
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Dog, Bovine
Background :
Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
ALTnames :
HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1
Immunogen :
Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.
Buffer :
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~32kDa by Western blot.
Application Summary :
FC, ICC, IHC (PS), WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.