GSK-3α/β monoclonal antibody (1H8) | Enzo Life Sciences ADI-KAM-ST002-D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

GSK-3α/β monoclonal antibody (1H8)

catalog :

ADI-KAM-ST002-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H8

The same clone is also sold as:

Santa Cruz Biotechnology: sc-56913
Novus Biologicals: NB110-85523

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 1

Reference
Knockaert M, Wieking K, Schmitt S, Leost M, Grant K, Mottram J, et al. Intracellular Targets of Paullones. Identification following affinity purification on immobilized inhibitor. J Biol Chem. 2002;277:25493-501 pubmed

product information

Product ID :

ADI-KAM-ST002

Catalog Number :

ADI-KAM-ST002-D

Product Name :

GSK-3α/β monoclonal antibody (1H8)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

11964410

Clone :

1H8

Isotype :

IgG2b

SPECIES REACTIVITY :

Human, Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Dog, Bovine

Background :

Glycogen Synthase Kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

ALTnames :

Glycogen synthase kinase-3α/β

Immunogen :

Recombinant Xenopus GSK-3β.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects bands of ~51 and ~47kDa by Western blot.

Application Summary :

ICC, IHC, WB

Storage :

-20°C