product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Cyclin D1 monoclonal antibody (5D4)
catalog :
ADI-KAM-CC200-E
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5D4
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
Enzo Life Sciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 4a
Tai Y, Tung L, Lin Y, Lu P, Chu P, Wang M, et al. Grb7 Protein Stability Modulated by Pin1 in Association with Cell Cycle Progression. PLoS ONE. 2016;11:e0163617 pubmed publisher
product information
Product ID :
ADI-KAM-CC200
Catalog Number :
ADI-KAM-CC200-E
Product Name :
Cyclin D1 monoclonal antibody (5D4)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µg
Image Legend 4 :
Western blot analysis of ZR- 75-1 (1), MCF7 (2), HeLa (3), WR19L (4) and NIH/3T3 (5) cells probed with Cyclin D1 mAb (5D4).
Clone :
5D4
Isotype :
IgG2a
SPECIES REACTIVITY :
Human, Mouse
Background :
Cyclins are a family of proteins which function as the regulatory subunits of cyclin/cyclin-dependent kinase (Cdk) holoenzymes, mediating entry into and progression through the cell cycle. Each cyclin is expressed in a temporal fashion during the cell cycle, partnering with a specific Cdk to activate its protein kinase activity. The most well defined mitotic cyclins are: cyclin D, which partners with cdks 4 and 6 during early G1 phase; cyclin E, which activates Cdk2 in the G1 to S phase transition; cyclin A, which utilizes Cdks 1 and 2 during progression through S phase and into G2; and cyclin B, which activates Cdk1 in M-phase promotion.
ALTnames :
CCND1
Immunogen :
Recombinant human cyclin D1.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol.
Uses :
Detects a band of ~36kDa by Western blot.
Application Summary :
FC, ICC, IHC (PS), IP, WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.