CaMKII monoclonal antibody (6G9) | Enzo Life Sciences ADI-KAM-CA002-F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

CaMKII monoclonal antibody (6G9)

catalog :

ADI-KAM-CA002-F

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6G9

application :

western blot, immunohistochemistry

citations: 2

Reference
Abbott A, Fissore R, Ducibella T. Identification of a translocation deficiency in cortical granule secretion in preovulatory mouse oocytes. Biol Reprod. 2001;65:1640-7 pubmed
Erondu N, Kennedy M. Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. J Neurosci. 1985;5:3270-7 pubmed

product information

Product ID :

ADI-KAM-CA002

Catalog Number :

ADI-KAM-CA002-F

Product Name :

CaMKII monoclonal antibody (6G9)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

200 µg

list of Pubmed id :

11717123, 4078628

Clone :

6G9

Isotype :

IgG1

SPECIES REACTIVITY :

Mouse, Rat

Background :

CaMKIIa, the alpha subunit of Ca2+ calmodulin-dependent protein kinase II is part of a family of multifunctional protein kinases, which play a major role in Ca2+-mediated signal transduction. CaMKIIa is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites as well as the soma of the neuron. The neuronal CaMKII consists of two major subunits of 52 and 60 kDa which are encoded by a- and b-CaMKII genes, respectively. Additional isoforms are generated by alternative splicing of these as well as of the ubiquitious g- and d-CaMKII genes. Each subunit has an ATP-binding domain (arginine-X-X-serune/threonine), consensus phosphorylation site, catalytic domain, and a centrally located regulatory domain which has calmodulin binding activity. Activation and autophosphorylation of CaMKII may regulate numerous neuronal processes which includes two forms of synaptic plasticity, long term potentiation and long term depression. Neuronal CaMKII subunits assemble as large multimeric holoenzymes. The C-terminal association domains of 6-12 subunits assemble into a central globular structure from which the N-terminal catalytic/regulatory domains extend radially like petals of a flower. The subunit composition of the rat forebrain CaMKII holoenzyme consists of heteromers composed of a and β subunits at a ratio of 2:1 and homomers composed of only a subunits. The association of CaMKII subunits leads to the positioning of their catalytic/regulatory domains in close proximity and the neighboring calmodulin-bound subunits cooperate to rapidly phosphorylate each other. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.

ALTnames :

Calcium/calmodulin-dependent protein kinase II

Immunogen :

Rat CaMKII (partially purified).

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~50-60kDa by Western blot.

Application Summary :

IHC, WB, in vitro Assay

Storage :

-20°C