HSP70 (low endotoxin) (human), (recombinant) | Enzo Life Sciences ADI-ESP-555-F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

HSP70 (low endotoxin) (human), (recombinant)

catalog :

ADI-ESP-555-F

quantity :

200 µg

citations: 5

Reference
Tsai T, Ho J, Liu M, Lee T, Lu M, Liu C, et al. Role of Exogenous Hsp72 on Liver Dysfunction during Sepsis. Biomed Res Int. 2015;2015:508101 pubmed publisher
Armijo G, Okerblom J, Cauvi D, Lopez V, Schlamadinger D, Kim J, et al. Interaction of heat shock protein 70 with membranes depends on the lipid environment. Cell Stress Chaperones. 2014;19:877-86 pubmed publisher
Dvoriantchikova G, Santos A, Saeed A, Dvoriantchikova X, Ivanov D. Putative role of protein kinase C in neurotoxic inflammation mediated by extracellular heat shock protein 70 after ischemia-reperfusion. J Neuroinflammation. 2014;11:81 pubmed publisher
Luong M, Zhang Y, Chamberlain T, Zhou T, Wright J, Dower K, et al. Stimulation of TLR4 by recombinant HSP70 requires structural integrity of the HSP70 protein itself. J Inflamm (Lond). 2012;9:11 pubmed publisher
Gao B, Tsan M. Endotoxin contamination in recombinant human heat shock protein 70 (Hsp70) preparation is responsible for the induction of tumor necrosis factor alpha release by murine macrophages. J Biol Chem. 2003;278:174-9 pubmed

product information

Product ID :

ADI-ESP-555

Catalog Number :

ADI-ESP-555-F

Product Name :

HSP70 (low endotoxin) (human), (recombinant)

Product Type :

Protein

Size :

200 µg

list of Pubmed id :

26221596, 24789271, 22448747, 12403778

Background :

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

ALTnames :

Heat shock protein 70, HspA1A, HspA1B, HSP72

Purity :

≥90% (SDS-PAGE; Western blot)

Buffer :

Liquid. In Dulbecco’s PBS.

Uses :

ATPase activity assay (positive). Western blot control.

Application Summary :

ELISA, WB, in vitro Assay, Activity assay

Storage :

-80°C