HO-1 (rat), ELISA kit | Enzo Life Sciences ADI-EKS-810A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

HO-1 (rat), ELISA kit

catalog :

ADI-EKS-810A

quantity :

96 wells

citations: 11

Reference
Tomonaga T, Izumi H, Yoshiura Y, Myojo T, Oyabu T, Lee B, et al. Assessment of Pulmonary Toxicity Induced by Inhaled Toner with External Additives. Biomed Res Int. 2017;2017:4245309 pubmed publisher
Kowalczyk A, JeleÅ„ A, Å»ebrowska M, Balcerczak E, GorÄ…ca A. BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats. Oxid Med Cell Longev. 2016;2016:2356853 pubmed publisher
Chuang J, Huang J, Tsai S, Sun H, Yang S, Chuang P, et al. FGF9-induced changes in cellular redox status and HO-1 upregulation are FGFR-dependent and proceed through both ERK and AKT to induce CREB and Nrf2 activation. Free Radic Biol Med. 2015;89:274-86 pubmed publisher
Ehrnthaller C, Flierl M, Perl M, Denk S, Unnewehr H, Ward P, et al. The molecular fingerprint of lung inflammation after blunt chest trauma. Eur J Med Res. 2015;20:70 pubmed publisher
Han F, Guo Y, Xu L, Hou N, Han F, Sun X. Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway. Cell Physiol Biochem. 2015;36:1480-90 pubmed
Connolly M, FernÃ¡ndez Cruz M, Navas J. Recovery of redox homeostasis altered by CuNPs in H4IIE liver cells does not reduce the cytotoxic effects of these NPs: an investigation using aryl hydrocarbon receptor (AhR) dependent antioxidant activity. Chem Biol Interact. 2015;228:57-68 pubmed publisher
Morimoto Y, Oyabu T, Horie M, Kambara T, Izumi H, Kuroda E, et al. Pulmonary toxicity of printer toner following inhalation and intratracheal instillation. Inhal Toxicol. 2013;25:679-90 pubmed publisher
Jain K, Suryakumar G, Prasad R, Ganju L. Upregulation of cytoprotective defense mechanisms and hypoxia-responsive proteins imparts tolerance to acute hypobaric hypoxia. High Alt Med Biol. 2013;14:65-77 pubmed publisher
Saeki I, Matsuura T, Hayashida M, Taguchi T. Ischemic preconditioning and remote ischemic preconditioning have protective effect against cold ischemia-reperfusion injury of rat small intestine. Pediatr Surg Int. 2011;27:857-62 pubmed publisher
Furukawa Y, Urano T, Minamimura M, Nakajima M, Okuyama S, Furukawa S. 4-Methylcatechol-induced heme oxygenase-1 exerts a protective effect against oxidative stress in cultured neural stem/progenitor cells via PI3 kinase/Akt pathway. Biomed Res. 2010;31:45-52 pubmed
Wang J, Ma H, Boor P, Ramanujam V, Ansari G, Khan M. Up-regulation of heme oxygenase-1 in rat spleen after aniline exposure. Free Radic Biol Med. 2010;48:513-8 pubmed publisher

product information

Product ID :

ADI-EKS-810A

Catalog Number :

ADI-EKS-810A

Product Name :

HO-1 (rat), ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26424114, 26160485, 25617484, 24102468, 23537263, 21113784, 20203419, 19969074

Product Description :

The HO-1 (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

SPECIES REACTIVITY :

Rat

Background :

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

ALTnames :

Heme oxygenase 1

Uses :

For the quantitative determination of rat HO-1 in cell lysates, plasma, serum, and tissue. Cited sample type includes bronchoalveolar lavage fluid.

Application Summary :

ELISA, Colorimetric detection