HO-1 (human), ELISA kit | Enzo Life Sciences ADI-EKS-800 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

HO-1 (human), ELISA kit

catalog :

ADI-EKS-800

quantity :

96 wells

citations: 11

Reference
Javkhedkar A, Banday A. Antioxidant resveratrol restores renal sodium transport regulation in SHR. Physiol Rep. 2015;3: pubmed publisher
CalÃ² L, Savoia C, Davis P, Pagnin E, Ravarotto V, Maiolino G. Relationship between NOX4 level and angiotensin II signaling in Gitelman's syndrome. Implications with hypertension. Int J Clin Exp Med. 2015;8:7487-96 pubmed
Padmini E, Tharani J. Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes. Environ Monit Assess. 2015;187:4147 pubmed publisher
Banday A, Lokhandwala M. Transcription factor Nrf2 protects renal dopamine D1 receptor function during oxidative stress. Hypertension. 2013;62:512-7 pubmed publisher
Song W, Zukor H, Lin S, Liberman A, Tavitian A, Mui J, et al. Unregulated brain iron deposition in transgenic mice over-expressing HMOX1 in the astrocytic compartment. J Neurochem. 2012;123:325-36 pubmed publisher
Song W, Zukor H, Lin S, Hascalovici J, Liberman A, Tavitian A, et al. Schizophrenia-like features in transgenic mice overexpressing human HO-1 in the astrocytic compartment. J Neurosci. 2012;32:10841-53 pubmed
Bao W, Rong S, Zhang M, Yu X, Zhao Y, Xiao X, et al. Plasma heme oxygenase-1 concentration in relation to impaired glucose regulation in a non-diabetic Chinese population. PLoS ONE. 2012;7:e32223 pubmed publisher
Bharucha A, Kulkarni A, Choi K, Camilleri M, Lempke M, Brunn G, et al. First-in-human study demonstrating pharmacological activation of heme oxygenase-1 in humans. Clin Pharmacol Ther. 2010;87:187-90 pubmed publisher
Kirino Y, Takeno M, Iwasaki M, Ueda A, Ohno S, Shirai A, et al. Increased serum HO-1 in hemophagocytic syndrome and adult-onset Still's disease: use in the differential diagnosis of hyperferritinemia. Arthritis Res Ther. 2005;7:R616-24 pubmed
Kravets A, Hu Z, Miralem T, Torno M, Maines M. Biliverdin reductase, a novel regulator for induction of activating transcription factor-2 and heme oxygenase-1. J Biol Chem. 2004;279:19916-23 pubmed
Philippidis P, Mason J, Evans B, Nadra I, Taylor K, Haskard D, et al. Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: antiinflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ Res. 2004;94:119-26 pubmed

product information

Product ID :

ADI-EKS-800

Catalog Number :

ADI-EKS-800

Product Name :

HO-1 (human), ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26221292, 22881289, 22875919, 22427825, 19956091, 15899048, 14988408, 14656926

Product Description :

The HO-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 2.5 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

SPECIES REACTIVITY :

Human

Background :

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

ALTnames :

Heme oxygenase 1

Uses :

For the quantitative determination of human heme oxygenase-1 in cell lysates and tissue extracts. Cited sample types include serum and plasma.

Application Summary :

ELISA, Colorimetric detection