Anti-HSP60 IgG/A/M (human), ELISA kit | Enzo Life Sciences ADI-EKS-650 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

Anti-HSP60 IgG/A/M (human), ELISA kit

catalog :

ADI-EKS-650

quantity :

96 wells

citations: 8

Reference
PapuÄ‡ E, Krupski W, Kurys Denis E, Rejdak K. Antibodies against small heat-shock proteins in Alzheimer's disease as a part of natural human immune repertoire or activation of humoral response?. J Neural Transm (Vienna). 2016;123:455-61 pubmed publisher
GaloviÄ‡ R, Flegar MeÅ¡triÄ‡ Z, Vidjak V, MatokanoviÄ‡ M, BariÅ¡iÄ‡ K. Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis. Clin Biochem. 2016;49:66-9 pubmed publisher
RabczyÅ„ski M, Fiodorenko Dumas Å, Mastej K, Dumas I, Adamiec R, Paprocka Borowicz M. A relationship between serological markers of chronic C. pneumoniae and CMV infection and hsp60 in patients with atherosclerotic carotid stenosis. Acta Biochim Pol. 2015;62:89-95 pubmed
Papuc E, Kurys Denis E, Krupski W, Rejdak K. Humoral response against small heat shock proteins in Parkinson's disease. PLoS ONE. 2015;10:e0115480 pubmed publisher
Bleotu C, Chifiriuc M, Pîrcălăbioru G, BerteÅŸteanu Å, Grigore R, Ruta S, et al. Significance of serum antibodies against HSP 60 and HSP 70 for the diagnostic of infectious diseases. Virulence. 2014;5:828-31 pubmed publisher
Dolasık I, Birtas Atesoglu E, Tarkun P, Mehtap O, Keski H, Dogru A, et al. Decreased serum heat shock protein 60 levels in newly diagnosed immune thrombocytopenia patients. Platelets. 2015;26:220-3 pubmed publisher
Adamus G, BONNAH R, Brown L, David L. Detection of autoantibodies against heat shock proteins and collapsin response mediator proteins in autoimmune retinopathy. BMC Ophthalmol. 2013;13:48 pubmed publisher
Peraçoli J, Bannwart Castro C, Romão M, Weel I, Ribeiro V, Borges V, et al. High levels of heat shock protein 70 are associated with pro-inflammatory cytokines and may differentiate early- from late-onset preeclampsia. J Reprod Immunol. 2013;100:129-34 pubmed publisher

product information

Product ID :

ADI-EKS-650

Catalog Number :

ADI-EKS-650

Product Name :

Anti-HSP60 IgG/A/M (human), ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26500006, 25654359, 25629316, 25483863, 24749912, 24066722, 24051131

Product Description :

This assay allows for reproducible, accurate and precise determination of IgG , IgA and IgM antibodies (total) to human Hsp60 in serum. The ELISA kit uses recombinant human Hsp60 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp60 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp60 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450 nm.

SPECIES REACTIVITY :

Human

Background :

Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.

Uses :

For the quantitative determination of anti-HSP60 IgG/A/M autoantibodies in human serum. Cited sample types include human plasma.

Application Summary :

ELISA, Colorimetric detection

Storage :

+4°C