HSP27 (human), ELISA kit | Enzo Life Sciences ADI-EKS-500 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

HSP27 (human), ELISA kit

catalog :

ADI-EKS-500

quantity :

96 wells

citations: 13

Reference
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Schuster C, Akslen L, Straume O. Expression of Heat Shock Protein 27 in Melanoma Metastases Is Associated with Overall Response to Bevacizumab Monotherapy: Analyses of Predictive Markers in a Clinical Phase II Study. PLoS ONE. 2016;11:e0155242 pubmed publisher
Thuringer D, Berthenet K, Cronier L, Solary E, Garrido C. Primary tumor- and metastasis-derived colon cancer cells differently modulate connexin expression and function in human capillary endothelial cells. Oncotarget. 2015;6:28800-15 pubmed publisher
Son S, Lee K, Chung J, Chang K, Roh H, Kim S, et al. Increased plasma levels of heat shock protein 70 associated with subsequent clinical conversion to mild cognitive impairment in cognitively healthy elderly. PLoS ONE. 2015;10:e0119180 pubmed publisher
Cui X, Xing J, Liu Y, Zhou Y, Luo X, Zhang Z, et al. COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study. Cell Stress Chaperones. 2015;20:473-81 pubmed publisher
Vangala R, Ravindran V, Ghatge M, Shanker J, Arvind P, Bindu H, et al. Integrative bioinformatics analysis of genomic and proteomic approaches to understand the transcriptional regulatory program in coronary artery disease pathways. PLoS ONE. 2013;8:e57193 pubmed publisher
Marquez E, SADOWSKI E, Reese S, Vidyasagar A, Artz N, Fain S, et al. Serum HSP27 is associated with medullary perfusion in kidney allografts. J Nephrol. 2012;25:1075-80 pubmed publisher
Hu Y, Yeh H, Tsao H, Tai C, Lin Y, Chang S, et al. Electrophysiological correlation and prognostic impact of heat shock protein 27 in atrial fibrillation. Circ Arrhythm Electrophysiol. 2012;5:334-40 pubmed publisher
Beyer I, Bautmans I, Njemini R, Demanet C, Bergmann P, Mets T. Effects on muscle performance of NSAID treatment with piroxicam versus placebo in geriatric patients with acute infection-induced inflammation. A double blind randomized controlled trial. BMC Musculoskelet Disord. 2011;12:292 pubmed publisher
Beyer I, Njemini R, Bautmans I, Demanet C, Mets T. Immunomodulatory effect of NSAID in geriatric patients with acute infection: effects of piroxicam on chemokine/cytokine secretion patterns and levels of heat shock proteins. A double-blind randomized controlled trial. (ISRCTN58517443). Cell Stress Chaperones. 2012;17:255-65 pubmed publisher
Wood K, Voss O, Huang Q, Parihar A, Mehta N, Batra S, et al. The small heat shock protein 27 is a key regulator of CD8+ CD57+ lymphocyte survival. J Immunol. 2010;184:5582-8 pubmed publisher
Hecker J, Sundram H, Zou S, Praestgaard A, Bavaria J, Ramchandren S, et al. Heat shock proteins HSP70 and HSP27 in the cerebral spinal fluid of patients undergoing thoracic aneurysm repair correlate with the probability of postoperative paralysis. Cell Stress Chaperones. 2008;13:435-46 pubmed publisher
Paulsen G, Vissing K, Kalhovde J, Ugelstad I, Bayer M, Kadi F, et al. Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans. Am J Physiol Regul Integr Comp Physiol. 2007;293:R844-53 pubmed

product information

Product ID :

ADI-EKS-500

Catalog Number :

ADI-EKS-500

Product Name :

HSP27 (human), ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

25768018, 25620081, 23468932, 22383348, 22354927, 22208783, 22057579, 20385876, 18418731, 17522120

Product Description :

The HSP27 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis. Highly sensitive quantitative determination of Hsp27 independent of phosphorylation state

SPECIES REACTIVITY :

Human

Background :

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-α, p70 S6K, PKD I, and PKC-δ. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

ALTnames :

Heat shock protein 27

Uses :

For the quantitative determination of human HSP27 in cell lysates, plasma, serum, and tissue.

Application Summary :

ELISA, Colorimetric detection