NFκB p65 ELISA kit | Enzo Life Sciences ADI-EKS-446 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

NFκB p65 ELISA kit

catalog :

ADI-EKS-446

quantity :

2 x 96 wells

citations: 11

Reference
Cleret Buhot A, Zhang Y, Planas D, Goulet J, Monteiro P, Gosselin A, et al. Identification of novel HIV-1 dependency factors in primary CCR4(+)CCR6(+)Th17 cells via a genome-wide transcriptional approach. Retrovirology. 2015;12:102 pubmed publisher
Zhang Y, He K, Wang F, Li X, Liu D. Notch-1 signaling regulates astrocytic proliferation and activation after hypoxia exposure. Neurosci Lett. 2015;603:12-8 pubmed publisher
Gülçubuk A, Haktanir D, Cakiris A, Ustek D, Guzel O, Erturk M, et al. The effects of resveratrol on tissue injury, oxidative damage, and pro-inflammatory cytokines in an experimental model of acute pancreatitis. J Physiol Biochem. 2014;70:397-406 pubmed publisher
Gülçubuk A, Haktanir D, Cakiris A, Ustek D, Guzel O, Erturk M, et al. Effects of curcumin on proinflammatory cytokines and tissue injury in the early and late phases of experimental acute pancreatitis. Pancreatology. 2013;13:347-54 pubmed publisher
de Bittencourt Pasquali M, Gelain D, Zeidán Chuliá F, Pires A, Gasparotto J, Terra S, et al. Vitamin A (retinol) downregulates the receptor for advanced glycation endproducts (RAGE) by oxidant-dependent activation of p38 MAPK and NF-kB in human lung cancer A549 cells. Cell Signal. 2013;25:939-54 pubmed publisher
Glinka Y, Mohammed N, Subramaniam V, Jothy S, Prud homme G. Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-?B activation and tumor sphere formation. Biochem Biophys Res Commun. 2012;425:775-80 pubmed publisher
Paimela T, Ryhanen T, Kauppinen A, Marttila L, Salminen A, Kaarniranta K. The preservative polyquaternium-1 increases cytoxicity and NF-kappaB linked inflammation in human corneal epithelial cells. Mol Vis. 2012;18:1189-96 pubmed
Zanotto Filho A, Braganhol E, Edelweiss M, Behr G, Zanin R, Schröder R, et al. The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma. J Nutr Biochem. 2012;23:591-601 pubmed publisher
Zanotto Filho A, Braganhol E, Schröder R, de Souza L, Dalmolin R, Pasquali M, et al. NF?B inhibitors induce cell death in glioblastomas. Biochem Pharmacol. 2011;81:412-24 pubmed publisher
Scherbakov A, Lobanova Y, Andreeva O, Shatskaya V, Krasil nikov M. Oestrogen treatment enhances the sensitivity of hormone-resistant breast cancer cells to doxorubicin. Biosci Rep. 2011;31:137-43 pubmed publisher
Zwart S, Pierson D, Mehta S, Gonda S, Smith S. Capacity of omega-3 fatty acids or eicosapentaenoic acid to counteract weightlessness-induced bone loss by inhibiting NF-kappaB activation: from cells to bed rest to astronauts. J Bone Miner Res. 2010;25:1049-57 pubmed publisher

product information

Product ID :

ADI-EKS-446

Catalog Number :

ADI-EKS-446

Product Name :

NFκB p65 ELISA kit

Product Type :

Kit

Size :

2 x 96 wells

list of Pubmed id :

26654242, 26182882, 23890132, 23333461, 22885184, 22605930, 21775121, 21040711, 20662767, 19874203

Product Description :

The NFκB p65 EIA kit provides a sensitive, rapid and reliable method to measure the active form of NFκB p65. The assay uses streptavidin-coated plates with bound NFκB biotinylated-consensus sequence to capture only the active form of NFκB. The captured active NFκB is incubated with a specific NFκB p65 antibody, which is then detected using an HRP conjugated secondary antibody. The assay is developed with a chemiluminescent substrate and the signal is detected using a luminometer. Higher throughput 96 well plate based method faster than EMSA and Western blot with sensitive chemiluminescent detection.

SPECIES REACTIVITY :

Human, Mouse, Rat

Background :

Stimulation of the canonical NFκB pathway leads to the phosphorylation of and subsequent degradation of IκB; phosphorylation of the latter is controlled by a specific set of proteins referred to as the I Kappa Kinases (IKK), which include IKKα and IKKβ. Under normal conditions, IκB acts as a key regulatory molecule in that it sequesters p65 or Rel A within the cytoplasm; this blocks NFκB dependent transcription. Upon phosphorylation of IκB by IKK’s, p65 is released and free to associate with the p50 subunit; p65 acts as a transactivator whereas p50 possesses DNA binding activity. Complex formation leads to the subsequent translocation of the NFκB complex into the nucleus and the concomitant up-regulation of NFκB dependent genes. Disruption of this signaling nexus has been documented in a number of human pathological conditions including inflammation, infection and cancer.

Uses :

For the quantitative determination of active NFκB p65 in cell lysates of human, mouse, and rat origin. Cited sample(s) include nuclear protein extracts.

Application Summary :

ELISA, Chemiluminescence