EGF receptor monoclonal antibody (6F1) | Enzo Life Sciences ADI-CSA-330-E product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

EGF receptor monoclonal antibody (6F1)

catalog :

ADI-CSA-330-E

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F1

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:800	Higa Nakamine S, Maeda N, Toku S, Yamamoto H. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem. 2015;290:25974-85 pubmed publisher
western blot; human; fig 2a	Nishi H, Maeda N, Izumi S, Higa Nakamine S, Toku S, Kakinohana M, et al. Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells. Eur J Pharmacol. 2015;748:133-42 pubmed publisher
	Noguchi N, Kondo Y, Maeda N, Higa Nakamine S, Toku S, Maruyama J, et al. Phosphorylation of epidermal growth factor receptor at serine 1047 by MAP kinase-activated protein kinase-2 in cultured lung epithelial cells treated with flagellin. Arch Biochem Biophys. 2013;529:75-85 pubmed publisher
	Higa Nakamine S, Maeda N, Toku S, Yamamoto T, Yingyuenyong M, Kawahara M, et al. Selective cleavage of ErbB4 by G-protein-coupled gonadotropin-releasing hormone receptor in cultured hypothalamic neurons. J Cell Physiol. 2012;227:2492-501 pubmed publisher

product information

Product ID :

ADI-CSA-330

Catalog Number :

ADI-CSA-330-E

Product Name :

EGF receptor monoclonal antibody (6F1)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

25542757, 23220022, 21898395

Clone :

6F1

Isotype :

IgG2b

SPECIES REACTIVITY :

Human

Background :

The epidermal growth factor receptor (EGFR) is a 170-kDa membrane bound receptor consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region, and a tyrosine kinase domain-containing cytoplasmic region. Ligand binding induces receptor homo- or heterodimerization, triggering autophosphorylation of cytoplasmic tyrosine residues that provide docking sites for Src homology 2 (SH2) domain-containing signaling molecules. Src-mediated phosphorylation of EGFR at Tyr845 may contribute to EGFR hyperactivity in cancer, while autophosphorylation of EGFR at Tyr1068 and/or Tyr1173 in response to ligand binding is known to recruit cofactors (e.g. SHP1, RasGAP, SOS1, Grb2) that activate pathways associated with cell growth, apoptosis, adhesion, receptor endocytosis, and protein degradation.

ALTnames :

EGFR, epidermal growth factor receptor

Immunogen :

Recombinant human EGF receptor (C-terminal fragment).

Buffer :

Lyophilized. In PBS, pH 7.2, containing 10mg/ml sucrose and 1mg/ml sodium azide. Reconstitute in 1 ml of distilled water.

Uses :

Detects a band of ~170kDa by Western blot.

Application Summary :

FC, ICC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage