This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Bad polyclonal antibody
catalog :
ADI-AAP-020-D
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
mouse, rat, dogs, bovine
application :
western blot, immunohistochemistry
product information
Product ID :
ADI-AAP-020
Catalog Number :
ADI-AAP-020-D
Product Name :
Bad polyclonal antibody
Host species :
Rabbit
Clonality :
Polyclonal
Product Type :
Antibody
Size :
50 µg
SPECIES REACTIVITY :
Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Drosophila, Bovine
Background :
Bad is an important regulatory protein involved in the control of cell survival and apoptosis, and is a member of the BH3-only family of proteins such as Bim and Bid that share homology with Bcl-2 only in the BH3 domain. Phosphorylated Bad is sequestered in the cytosol by 14-3-3 proteins under normal conditions, but is dephosphorylated and activated following apoptotic signals. Apoptotic stimuli induce Bad dephosphorylation by a number of phosphatases (e.g., calcineurin) and recruitment to the mitochondria, where Bad heterodimerizes with and inhibits prosurvival proteins of the Bcl family. In contrast, survival signals lead to phosphorylation of Bad at multiple serine residues, including phosphorylation at Ser112 and Ser136 which induce association with 14-3-3 proteins. Phosphorylation at Ser112 and Ser136 is required for a third phosphorylation event at Ser155, which induces dissociation of Bad/Bcl heterodimers to promote cell survival. This antibody specifically binds the Bad protein, but does not recognize Bad/Bcl-2 heterodimers in immunoprecipitation assays.
ALTnames :
BCL2 antagonist of cell death
Immunogen :
Synthetic peptide corresponding to a portion of mouse Bad.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~23kDa by Western blot.
Application Summary :
IHC, WB
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.