Caspase-9 monoclonal antibody (5B4) | Enzo Life Sciences ADI-AAM-139-E product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Caspase-9 monoclonal antibody (5B4)

catalog :

ADI-AAM-139-E

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B4

reactivity :

human, mouse, rat

application :

western blot

citations: 5

Reference
Yi S, Moore C, Lee R. Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis. Apoptosis. 2007;12:1183-93 pubmed
Jia L, Patwari Y, Kelsey S, Srinivasula S, Agrawal S, Alnemri E, et al. Role of Smac in human leukaemic cell apoptosis and proliferation. Oncogene. 2003;22:1589-99 pubmed
Degenhardt K, Sundararajan R, Lindsten T, Thompson C, White E. Bax and Bak independently promote cytochrome C release from mitochondria. J Biol Chem. 2002;277:14127-34 pubmed
Liu J, Opipari A, Tan L, Jiang Y, Zhang Y, Tang H, et al. Dysfunctional apoptosome activation in ovarian cancer: implications for chemoresistance. Cancer Res. 2002;62:924-31 pubmed
Huang L, Mivechi N, Moskophidis D. Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene. Mol Cell Biol. 2001;21:8575-91 pubmed

product information

Product ID :

ADI-AAM-139

Catalog Number :

ADI-AAM-139-E

Product Name :

Caspase-9 monoclonal antibody (5B4)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

17245639, 12642862, 11836241, 11830553, 11713291

Clone :

5B4

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Drosophila

Background :

Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signalling pathways that lead to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells, and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-9, also known as ICE-LAP61, Mch-62 or Apaf-33 is a 45 kDa protein that is involved in one of the relatively well characterized caspase cascades. It is triggered by Cytochrome C release from the mitochondria, which promotes the activation of caspase-9 by forming a complex with Apaf-1 in the presence of dATP. Once activated, caspase-9 initiates a caspase cascade involving caspase-3, -6, and -7. The active form of caspase-9 is composed of a 32-35 kDa subunit and 10 kDa subunit. Caspase-9 is expressed in a variety of tissues.

Immunogen :

Recombinant human caspase-9 (N-terminal fragment).

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol.

Uses :

Detects a band of ~46-50kDa by Western blot.

Application Summary :

Storage :

-20°C