Caspase-8 monoclonal antibody (5F7) | Enzo Life Sciences ADI-AAM-118-E product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Caspase-8 monoclonal antibody (5F7)

catalog :

ADI-AAM-118-E

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F7

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1c	Park S, Jwa E, Shin S, Ju E, Park I, Pak J, et al. Ibulocydine sensitizes human hepatocellular carcinoma cells to TRAIL-induced apoptosis via calpain-mediated Bax cleavage. Int J Biochem Cell Biol. 2017;83:47-55 pubmed publisher
western blot; human; fig 3, 4	Crowder R, Dicker D, El Deiry W. The Deubiquitinase Inhibitor PR-619 Sensitizes Normal Human Fibroblasts to Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-mediated Cell Death. J Biol Chem. 2016;291:5960-70 pubmed publisher
	Estornes Y, Toscano F, Virard F, Jacquemin G, Pierrot A, Vanbervliet B, et al. dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8. Cell Death Differ. 2012;19:1482-94 pubmed publisher
	Son Y, Kim E, Kim J, Kim S, Kwon T, Yoon A, et al. Silibinin sensitizes human glioma cells to TRAIL-mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin. Cancer Res. 2007;67:8274-84 pubmed
	Wong R, Tsang W, Chau P, Co N, Tsang T, Kwok T. p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3. Mol Cancer Ther. 2007;6:1054-61 pubmed
	Austin C, Lawrence D, Peden A, Varfolomeev E, Totpal K, De MaziÃ¨re A, et al. Death-receptor activation halts clathrin-dependent endocytosis. Proc Natl Acad Sci U S A. 2006;103:10283-10288 pubmed publisher
	Varfolomeev E, Maecker H, Sharp D, Lawrence D, Renz M, Vucic D, et al. Molecular determinants of kinase pathway activation by Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand. J Biol Chem. 2005;280:40599-608 pubmed
	Hyer M, Croxton R, Krajewska M, Krajewski S, Kress C, Lu M, et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005;65:4799-808 pubmed
	Murphy B, Creagh E, Martin S. Interchain proteolysis, in the absence of a dimerization stimulus, can initiate apoptosis-associated caspase-8 activation. J Biol Chem. 2004;279:36916-22 pubmed
	Dirsch V, Müller I, Eichhorst S, Pettit G, Kamano Y, Inoue M, et al. Cephalostatin 1 selectively triggers the release of Smac/DIABLO and subsequent apoptosis that is characterized by an increased density of the mitochondrial matrix. Cancer Res. 2003;63:8869-76 pubmed
	Menard C, Wagner M, Ruzsics Z, Holak K, Brune W, Campbell A, et al. Role of murine cytomegalovirus US22 gene family members in replication in macrophages. J Virol. 2003;77:5557-70 pubmed
	Barilà D, Rufini A, Condo I, Ventura N, Dorey K, Superti Furga G, et al. Caspase-dependent cleavage of c-Abl contributes to apoptosis. Mol Cell Biol. 2003;23:2790-9 pubmed

product information

Product ID :

ADI-AAM-118

Catalog Number :

ADI-AAM-118-E

Product Name :

Caspase-8 monoclonal antibody (5F7)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

22421964, 17804742, 17363498, 16801533, 16227629, 15930300, 15210716, 14695204, 12719548, 12665579

Image Legend 4 :

Western Blot analysis of Caspase-8 fragments in apoptosis induced Jurkat cells using Caspase-8, mAb (5F7) (Prod. No. ADI-AAM-118). The Jurkat cells were treated with a FAS monoclonal antibody for the indicated time

Clone :

5F7

Isotype :

IgG2b

SPECIES REACTIVITY :

Human

Background :

Caspase-8/FADD-like ICE (FLICE), is an ~55 kDa cytosolic protein belonging to the Fas/APO-1/CD95 death-inducing signaling complex. Although researchers have defined eight different isoforms of caspase-8 at the mRNA level (8/a-h), significant expression of only Caspase-8/a and /b occurs at the protein level in a variety of cell lines. Caspase-8 contains two death effector domains (DEDs) and exhibits sequence homology to both FADD, a signal transducer of Fas-induced apoptosis, and to the ICE/CED-3 family of cysteine proteases. Caspase-8 binds to FADD through its amino terminal DEDs and induces apoptosis when overexpressed. Activation of Caspase-8 involves a two-step proteolysis whereby the cleavage of Caspase-8 generates a 43 kDa fragment (p43) and a 12 kDa fragment further processed to 10 kDa, then the cleavage of receptor-associated p43 yields p26 and the release of the active site containing p18. A number of cytosolic factors inhibit the FADD-Caspase-8 containing signaling complex, including the ICE/CED-3 family inhibitor CrmA which inhibits Caspase-8 protease activity, and the short and long forms of FLIP that bind to the FADD-Caspase-8 complex.

ALTnames :

FLICE

Immunogen :

C-terminal fragment of recombinant human caspase-8 (amino acids 176-460).

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol.

Uses :

Detects a band of ~55-57kDa by Western blot. Also detects the cleaved intermediate form (p41/43) and the activated large caspase-8 subunit p18 after apoptosis induction.

Application Summary :

IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage