This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
5-Hydroxymethylcytosine polyclonal antibody
catalog :
ADI-905-904-0200
quantity :
200 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
methylated
application :
western blot, ELISA, immunoprecipitation
product information
Product ID :
ADI-905-904
Catalog Number :
ADI-905-904-0200
Product Name :
5-Hydroxymethylcytosine polyclonal antibody
Host species :
Rabbit
Clonality :
Polyclonal
Antigen modification :
Methylated
Product Type :
Antibody
Size :
200 µg
Isotype :
IgG1
SPECIES REACTIVITY :
Species independent
Background :
Recent advances in the field of epigenetics have identified 5-hydroxymethylcytosine (5-hmC) as a key factor in the regulation of gene expression, with substantial implications in the study of tissue differentiation, neurological development, and carcinogenesis. Studies of this epigenetic marker are typically confounded by a lack of reliable methodology for differentiation from the highly prevalent 5-methylcytosine in a DNA sample.  The 5-hydroxymethylcytosine pAb has been developed in order to robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA. Specificity of the antibody is enhanced such that crossreactivity with unmodified and methylated templates is suppressed to near-background levels. The antibody has been extensively tested and validated in ELISA and immunoprecipitation-based enrichment assays, and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting. Overall, the 5-hydroxymethylcytosine pAb has low cross reactivity with cytosine and 5-methylcytosine versus other available antibodies; can be used with a variety of genomic DNA sources; and has high sensitivity to low masses of 5-hydroxymethylcytosine DNA.
ALTnames :
5-hmC
Immunogen :
5-Hydroxymethylcytosine
Specificity :
Recognizes 5-hydroxymethylcytosine in single-stranded DNA from any mammalian, plant, insect, or microbial sources, as well as artificial templates used for standardization.
Buffer :
Liquid. In PBS, pH 7.5, containing 0.05% sodium azide.
Application Summary :
ELISA, IP, Immunoblot
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.