[pSer473]Akt polyclonal antibody | Enzo Life Sciences ADI-905-773-100 product information

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product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

[pSer473]Akt polyclonal antibody

catalog :

ADI-905-773-100

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot

product information

Product ID :

ADI-905-773

Catalog Number :

ADI-905-773-100

Product Name :

[pSer473]Akt polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Antigen modification :

Phosphorylated

Product Type :

Antibody

Size :

100 µg

SPECIES REACTIVITY :

Mouse

Background :

The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3β, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

ALTnames :

PKB, Protein kinase B, RAC

Immunogen :

Synthetic peptide corresponding to a portion of human Akt1 phosphorylated at Ser473.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~60kDa by Western blot.

Application Summary :

Storage :

-20°C