Direct 8-iso-PGF2α ELISA kit | Enzo Life Sciences ADI-901-091 product information

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product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

Direct 8-iso-PGF2α ELISA kit

catalog :

ADI-901-091

quantity :

5 x 96 wells

citations: 10

Reference
Johnston P, McCance D, Holmes V, Young I, McGinty A. Placental antioxidant enzyme status and lipid peroxidation in pregnant women with type 1 diabetes: The effect of vitamin C and E supplementation. J Diabetes Complications. 2016;30:109-14 pubmed publisher
Duffy M, O Kane C, Stevenson M, Young I, Harkin D, Mullan B, et al. A randomized clinical trial of ascorbic acid in open abdominal aortic aneurysm repair. Intensive Care Med Exp. 2015;3:50 pubmed publisher
Kumar R, Kohli S, Ali Z, Duhan K, Ram R, Gupta M, et al. CYBA (p22phox) variants associate with blood pressure and oxidative stress markers in hypertension: a replication study in populations of diverse altitudes. Hypertens Res. 2015;38:498-506 pubmed publisher
Li P, Tong Y, Yang H, Zhou S, Xiong F, Huo T, et al. Mitochondrial translocation of human telomerase reverse transcriptase in cord blood mononuclear cells of newborns with gestational diabetes mellitus mothers. Diabetes Res Clin Pract. 2014;103:310-8 pubmed publisher
Fang H, Lin W. Lipid peroxidation products do not activate hepatic stellate cells. Toxicology. 2008;253:36-45 pubmed publisher
Puthucheary S, Nathan S. Comparison of serum F2 isoprostane levels in diabetic patients and diabetic patients infected with Burkholderia pseudomallei. Singapore Med J. 2008;49:117-20 pubmed
Jeanes Y, Hall W, Proteggente A, Lodge J. Cigarette smokers have decreased lymphocyte and platelet alpha-tocopherol levels and increased excretion of the gamma-tocopherol metabolite gamma-carboxyethyl-hydroxychroman (gamma-CEHC). Free Radic Res. 2004;38:861-8 pubmed
Patel M, Bhat H. Differential oxidant potential of carcinogenic and weakly carcinogenic estrogens: Involvement of metabolic activation and cytochrome P450. J Biochem Mol Toxicol. 2004;18:37-42 pubmed
Hoffman Goetz L, Quadrilatero J. Treadmill exercise in mice increases intestinal lymphocyte loss via apoptosis. Acta Physiol Scand. 2003;179:289-97 pubmed
Bhat H, Calaf G, Hei T, Loya T, Vadgama J. Critical role of oxidative stress in estrogen-induced carcinogenesis. Proc Natl Acad Sci U S A. 2003;100:3913-8 pubmed

product information

Product ID :

ADI-901-091

Catalog Number :

ADI-901-091

Product Name :

Direct 8-iso-PGF2α ELISA kit

Product Type :

Kit

Size :

5 x 96 wells

list of Pubmed id :

26597598, 26215814, 25787042, 24480248, 18789371, 18301838, 15493460, 14994278, 14616245, 12655060

Product Description :

The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. The is a colorimetric competitive immunoassay kit for the quantitative determination of 8-iso-Prostaglandin F2α in biological fluids such as plasma, serum and tissue. To measure isoprostane in urine or culture supernatants, use the 8-iso-PGF2α EIA Kit. 8-iso-PGF2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF2α has been shown to circulate in plasma and is excreted in urine.

SPECIES REACTIVITY :

Species independent

Background :

Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia.

ALTnames :

8-iso-Prostaglandin F2α

Uses :

For the quantitative determination of 8-iso-PGF2α in plasma, serum, and tissue from any species. Cited sample types include cell lysate and urine.

Application Summary :

ELISA, Colorimetric detection

Storage :

+4°C