HSF1 ELISA kit | Enzo Life Sciences ADI-900-198 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

HSF1 ELISA kit

catalog :

ADI-900-198

quantity :

96 wells

citations: 2

Reference
Goetzl E, Boxer A, Schwartz J, Abner E, Petersen R, Miller B, et al. Low neural exosomal levels of cellular survival factors in Alzheimer's disease. Ann Clin Transl Neurol. 2015;2:769-73 pubmed publisher
Islam A, Abraham P, Hapner C, Andrews Shigaki B, Deuster P, Chen Y. Heat exposure induces tissue stress in heat-intolerant, but not heat-tolerant, mice. Stress. 2013;16:244-53 pubmed publisher

product information

Product ID :

ADI-900-198

Catalog Number :

ADI-900-198

Product Name :

HSF1 ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26273689, 22632404

Product Description :

The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 in cell lysates and tissue extracts from human and mouse origins.

SPECIES REACTIVITY :

Human

Background :

HSF1 belongs to a family of heat shock transcription factors (HSFs) that bind to highly conserved heat shock elements (HSEs) in the promoter regions of heat shock genes and regulate the expression of heat shock proteins (Hsps). Most HSFs have several common functional motifs including an N-terminal DNA binding domain essential for binding to the HSE and adjacent hydrophobic repeats essential for the formation of active trimers. Another short hydrophobic repeat located towards the C-terminal region of most HSFs is thought to be necessary for suppression of trimerization except in the case of yeast HSF and human HSF4. In higher eukaryotes, HSF1 is predominantly found in a diffuse cytoplasmic and nuclear distribution in unstressed cells. On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleo-plasmic distribution. HSF1 is post-translationally modified by both phosphorylation and sumoylation. Inducible phosphorylation at Ser230, Ser326, and Ser419 promote HSF1 activity, while constitutive phosphorylation sites at Ser303, Ser307, and Ser308 are inhibitory.

ALTnames :

Heat shock transcription factor 1

Uses :

For the quantitative determination of human and mouse HSF1 in cell lysates and tissue.

Application Summary :

ELISA, Colorimetric detection

Storage :

-20°C