[pSer473/474]Akt1/2 ELISA kit | Enzo Life Sciences ADI-900-162 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

[pSer473/474]Akt1/2 ELISA kit

catalog :

ADI-900-162

quantity :

96 wells

product information

Product ID :

ADI-900-162

Catalog Number :

ADI-900-162

Product Name :

[pSer473/474]Akt1/2 ELISA kit

Product Type :

Kit

Size :

96 wells

Product Description :

The [pSer473/474]Akt1/2 EIA kit is a colorimetric immunometric enzyme immunoassay kit. Absorbance is read at 450 nm. This is an ultra-sensitive assay that lets you measure as little as 5.5 pg/mL Akt1 or Akt2 phosphorylated on Ser473 or Ser474. Obtain fully quantitative results from up to 41 samples in duplicate in just 3 hours. The assay has been validated with up and down regulation experiments and compared to Western blot.

SPECIES REACTIVITY :

Human, Mouse, Rat

Background :

The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473/474 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3β, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

ALTnames :

PKB, Protein kinase B

Uses :

For the quantitative determination of [pSer473/474]Akt1/2 in cell lysates of human, mouse, and rat origin.

Application Summary :

ELISA, Colorimetric detection