[pThr180/Tyr182]p38 ELISA kit | Enzo Life Sciences ADI-900-101 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

[pThr180/Tyr182]p38 ELISA kit

catalog :

ADI-900-101

quantity :

96 wells

citations: 2

Reference
Choi K, Cho S, Kook S, Chun S, Bhattarai G, Poudel S, et al. Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L. in experimental ulcerative colitis models. J Ethnopharmacol. 2016;179:55-65 pubmed publisher
Kook S, Choi K, Cho S, Cho H, Lee K, Lee J. Catechin-7-O-Î²-D-glucopyranoside isolated from the seed of Phaseolus calcaratus Roxburgh ameliorates experimental colitis in rats. Int Immunopharmacol. 2015;29:521-527 pubmed publisher

product information

Product ID :

ADI-900-101

Catalog Number :

ADI-900-101

Product Name :

[pThr180/Tyr182]p38 ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26721217, 26453508

Product Description :

The [pThr180/Tyr182]p38 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Use less precious sample per test compared to Western blot with higher throughput sample analysis; measure up to 40 samples in duplicate per kit.

SPECIES REACTIVITY :

Human, Mouse

Background :

Six proteins of the p38 subfamily of MAP kinases have been identified to date, including: p38α (SAPK2, CSBP) and its splice variant Mxi; p38β and β2; p38γ (ERK6, SAPK3); and p38δ (SAPK4). p38 MAP kinases contain a conserved TGY motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-inflammatory responses. MKK3, MKK4, and MKK6 are known to activate p38 family members with varying specificity. All four p38 isoforms are able to phosphorylate the activating transcription factor 2 (ATF2), and differentially activate numerous substrates including Mnk1/2, Msk, phospholipase A2, and the MAPKAP kinase-2 and -3. Activated MAPKAP kinase-2 subsequently phosphorylates the heat shock protein Hsp27, an F-actin binding protein involved in cytoskeletal maintenance.

ALTnames :

SAPK2, SAP kinase 2

Uses :

For the quantitative determination of [pThr180/Tyr182]p38 in cell lysates of human and mouse origin.

Application Summary :

ELISA, Colorimetric detection