[pThr202/Tyr204]Erk1/2 ELISA kit | Enzo Life Sciences ADI-900-098A-0001 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

[pThr202/Tyr204]Erk1/2 ELISA kit

catalog :

ADI-900-098A-0001

quantity :

96 wells

citations: 6

Reference
Kim J, Ma T. Autocrine fibroblast growth factor 2-mediated interactions between human mesenchymal stem cells and the extracellular matrix under varying oxygen tension. J Cell Biochem. 2013;114:716-27 pubmed publisher
Arita N, Pelaez D, Cheung H. Activation of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) is needed for the TGF?-induced chondrogenic and osteogenic differentiation of mesenchymal stem cells. Biochem Biophys Res Commun. 2011;405:564-9 pubmed publisher
Fuqua C, Akomeah R, Price J, Adunyah S. Involvement of ERK-1/2 in IL-21-induced cytokine production in leukemia cells and human monocytes. Cytokine. 2008;44:101-7 pubmed publisher
Chernyavsky A, Arredondo J, Karlsson E, Wessler I, Grando S. The Ras/Raf-1/MEK1/ERK signaling pathway coupled to integrin expression mediates cholinergic regulation of keratinocyte directional migration. J Biol Chem. 2005;280:39220-8 pubmed
Bottone F, Moon Y, Alston Mills B, Eling T. Transcriptional regulation of activating transcription factor 3 involves the early growth response-1 gene. J Pharmacol Exp Ther. 2005;315:668-77 pubmed
Mahapatra N, Mahata M, O Connor D, Mahata S. Secretin activation of chromogranin A gene transcription. Identification of the signaling pathways in cis and in trans. J Biol Chem. 2003;278:19986-94 pubmed

product information

Product ID :

ADI-900-098A

Catalog Number :

ADI-900-098A-0001

Product Name :

[pThr202/Tyr204]Erk1/2 ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

23060043, 21262199, 18707899, 16150734, 16079301, 12646581

Product Description :

The [pThr202/Tyr204]Erk1/2 EIA kit is a complete assay for the quantitative determination of phosphorylated Erk1/2 using colorimetric detection read at 450 nm. The Extracellular regulated kinases, Erk-1 and Erk-2, both play a critical role in cellular proliferation. These extracellular kinases, are known to reside downstream of the Mitogen activated protein kinase/Extracellular protein kinases referred to as MEK. Both MEK-1 and MEK-2 phosphorylate and activate Erk on the conserved TEY motif within the activation loop of the kinase.

SPECIES REACTIVITY :

Human, Mouse, Rat

Background :

The mitogen-activated protein kinase (MAPK) pathway exists in all eukaryotes. It consists of several subgroups including ERK 1/2 (extracellular signal-regulated kinase), JNK and p38 kinases. These kinases regulate and control fundamental cellular processes including proliferation, apoptosis, survival and differentiation. They are activated by diverse stimuli including cytokines, growth factors, irradiation, changes in osmolarity, heat and shear stress. MAP kinases are characterized by their requirement for dual phosphorylation for full activation. ERK1 (44kDa) and ERK2 (42kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif. The literature contains numerous and extensive reviews on MAP kinase.

ALTnames :

MAPK2/3, MAP kinase 2/3

Uses :

For the measurement of [pThr202/Tyr204]Erk1/2 in cell lysates of human, mouse, and rat origin.

Application Summary :

ELISA, Colorimetric detection