CD40L (soluble) (human), (recombinant)
Enzo Life Sciences
catalog: ALX-522-015-2010
citations: 10

Figure 2: Stimulation of B cells by CD40L, Soluble (human) (rec.). Dose dependent costimulation of CD19+ cells by CD40L in the presence of 10ng/ml of IL-4 and of 2µg/ml anti-µ antibodies. Note that in the presence of enhancer (Prod. No. ALX-804-034), the activity of CD40L is increased >1'000 fold.Method: CD19+ cells were incubated in 96-well plates (105 cells/well in 100µl RPMI supplemented with 10% FCS) for 72 hours in the presence of 10ng/ml IL-4, 2µg/ml of goat anti-human µ chain antibody and with the indicated concentration of CD40L in the presence and absence of 1µg/ml enhancer (Prod. No. ALX-804-034). Cells were pulsed for an additional 6 hours with [3H]thymidine (1µCi/well) and harvested. [3H]thymidine incorporation was monitored by liquid scintillation counting.

Schematic structure of human CD40L (peptide, aa. 116 to 261).
quantity: 2 x 10 µg
price:
to the supplier
CD40L (soluble) (human), (recombinant)
Enzo Life Sciences
catalog: ALX-522-015-6010
citations: 10

Figure 2: Stimulation of B cells by CD40L, Soluble (human) (rec.). Dose dependent costimulation of CD19+ cells by CD40L in the presence of 10ng/ml of IL-4 and of 2µg/ml anti-µ antibodies. Note that in the presence of enhancer (Prod. No. ALX-804-034), the activity of CD40L is increased >1'000 fold.Method: CD19+ cells were incubated in 96-well plates (105 cells/well in 100µl RPMI supplemented with 10% FCS) for 72 hours in the presence of 10ng/ml IL-4, 2µg/ml of goat anti-human µ chain antibody and with the indicated concentration of CD40L in the presence and absence of 1µg/ml enhancer (Prod. No. ALX-804-034). Cells were pulsed for an additional 6 hours with [3H]thymidine (1µCi/well) and harvested. [3H]thymidine incorporation was monitored by liquid scintillation counting.

Schematic structure of human CD40L (peptide, aa. 116 to 261).
quantity: 6 x 10 µg
price:
to the supplier
CD40L (soluble) (human), (recombinant)
Enzo Life Sciences
catalog: ALX-522-015-C010
citations: 10
quantity: 10 µg
price:
to the supplier
CD40L (soluble) (mouse), (recombinant)
Enzo Life Sciences
catalog: ALX-522-070-2010
citations: 1
quantity: 2 x 10 µg
price:
to the supplier
Fc (human):CD40L (soluble) (mouse), (recombinant)
Enzo Life Sciences
catalog: ALX-522-072-C010


SDS-PAGE analysis of Fc (human):CD40L (soluble) (mouse), (recombinant) (Prod. no. ALX-522-072): Lane 1: MW Marker, Lane 2: 1 µg.

Figure: Proliferation of mouse B cells induced by Fc (human):CD40L, Soluble (mouse) (rec.) (Prod. No. ALX-522-072). Method: Splenocytes from Balb/c mice were purified on B220 magnetic beads using the MACS system. The resulting B cells were put in culture at a cell density of 70'000 cells/well in a 96-well plate. Cells were activated with the indicated concentrations of Fc (human):CD40L, Soluble (mouse) (rec.). After 36 hours, 0.5µCi of 3H-Thymidine/well was added. Cells were pulsed for 10 hours, then freeze-thawed, harvested and counted.
quantity: 10 µg
price:
to the supplier
Fc (human):CD40L (soluble) (human), (recombinant)
Enzo Life Sciences
catalog: ALX-522-076-C010


Figure: Proliferation of human dendritic cells (DC) induced by Fc (human):CD40L, Soluble (human) (rec.) (Prod. No. ALX-522-076).Method: Human DC were derived from GMCSF/IL-4 treated monocytes. The so obtained DC, 105 cells/assay, were incubated with 3µg/ml Fc (human):CD40L, Soluble (human) (rec.) (A), 3µg/ml boiled Fc (human):CD40L, Soluble (human) (rec.) (B), 3µg/ml control:Fc (C) and media (D). After 18 hours, DC maturation was analyzed by immunostaining with a CD83-specific MAb and cytofluorimetry analysis. Indicated are the percentages of activated DC.
quantity: 10 µg
price:
to the supplier