TRAIL (human) polyclonal antibody
Enzo Life Sciences
catalog: ALX-210-732-R100

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 3: Immunostaining of HEK 293T cells transfected with a expression plasmid coding for human TRAIL (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells as indicated, cells were fixed with 4% formaldehyde or acetone for 5 min. at RT. Slides were blocked with IgG, and incubated for 1 hour with PAb to TRAIL (Prod. No. ALX-210-732) at a dilution of 1:100 or control rabbit IgG in PBS containing 1% BSA. After washes with PBS, samples were incubated with a biotinylated secondary antibody to rabbit IgG for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

Figure 2: Detection of recombinant human TRAIL (Prod. No. ALX-522-003 ) but not of recombinant human FasL (Prod. No. ALX-522-001). Method: Recombinant soluble TRAIL, and recombinant soluble FasL as negative control, were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and detected with PAb to TRAIL (human) (Prod. No. ALX-210-732) at a dilution of 1:500. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.
quantity: 100 µl
price:
to the supplier

TRAIL (human) monoclonal antibody (2E5)
Enzo Life Sciences
catalog: ALX-804-296-C100

mouse monoclonal (2E5)
reactivity: human
application: flow cytometry
citations: 3

Figure: Neutralizing effect of MAb to TRAIL (human) (2E5) on TRAIL-induced apoptosis (TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018); containing Enhancer for Ligands).

Figure 2:  MAb to TRAIL (human) (2E5) (Prod. No. ALX-804-296) can be used to detect surface human TRAIL in transiently transfected 293T cells in flow cytometry as well on untransfected cells expressing endogenous surface TRAIL.Method: 293T cells (10cm dishes) were transfeced with 0, 1, or 15µg plasmid coding for full length human TRAIL for 24 hours using a standard calcium phosphate transfection method. All dishes were cotransfected with 1µg plasmid coding for EGFPas a cotransfection marker. Viable cells (FSC/SSC region R1), that were GFP-positive (transfected) (FL1 region R3) were analyzed with respect to surface expression of human TRAIL. Cells were stained with MAb to TRAIL (human) (2E5) as well as with an isotype-matched control (control IgG1) at 10µg/ml.  Binding of the primary MAbs is detected using RPE conjugated goat anti-mouse IgG (FL2).
quantity: 100 µg
price:
to the supplier

TRAIL (human) monoclonal antibody (III6F)
Enzo Life Sciences
catalog: ALX-804-326-C100

mouse monoclonal (III6F)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 5

quantity: 100 µg
price:
to the supplier

TRAIL (human) monoclonal antibody (HS501)
Enzo Life Sciences
catalog: ALX-804-300-C100

mouse monoclonal (HS501)
reactivity: human
application: western blot
citations: 1

Figure 2: Equal numbers of CV1-EBNA cells transfected with control expression plasmids (negative for human TRAIL) were analyzed and compared to a positive control cell-lysate using HS501.Method: CV1-EBNA cells, either transfected with pcDNA3 expression vector encoding TRAIL-R1 to TRAIL-R4 (lanes 1-4), or with human TRAIL (lane 5) were analyzed with HS501 MAb against TRAIL (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by a horseradish peroxidase conjugated anti-mouse IgG1 antibody and developed by ECL.

Figure 1: Equal numbers of Jurkat cells treated and untreated with IFNa compared with equal numbers of CV1-EBNA cells transfected with human TRAIL and a control expression plasmid (not encoding hTRAIL) were analyzed by using HS501 (Prod. No. ALX-804-300.Method: Jurkat cells were treated with IFNa [1000 IU/ml] for 8 hours (lane 1) or were left untreated (lane 2), and CV1-EBNA cells were transfected with pcDNA3 expression vector encoding human TRAIL (lane 3), or with human TRAIL-R1 (lane 4). Lysates of equal numbers of cells were analyzed by Western blot with anti-TRAIL HS501 (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by horseradish peroxidase-conjugated anti-mouse IgG1 antibody and developed by ECL.
quantity: 100 µg
price:
to the supplier

TRAIL (human) monoclonal antibody (VI10E)
Enzo Life Sciences
catalog: ALX-804-325-C100

mouse monoclonal (VI10E)
reactivity: human
application: ELISA, immunoprecipitation, flow cytometry
citations: 1

quantity: 100 µg
price:
to the supplier

TRAIL (human) polyclonal antibody
Enzo Life Sciences
catalog: ADI-AAP-470-E

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1µg/ml.

Immunohistochemistry analysis of TRAIL in human brain tissue with TRAIL antibody at 20µg/ml.
quantity: 100 µg
price:
to the supplier

TRAIL (human) polyclonal antibody
Enzo Life Sciences
catalog: BML-SA259-0100

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 µg
price:
to the supplier

TRAIL (human) monoclonal antibody (RIK-2)
Enzo Life Sciences
catalog: ALX-804-907-0100

mouse monoclonal (RIK-2)
reactivity: human
application: flow cytometry
citations: 2

quantity: 100 µg
price:
to the supplier

TRAIL-R3 (human) monoclonal antibody (DJR3)
Enzo Life Sciences
catalog: ALX-804-908-0100

mouse monoclonal (DJR3)
reactivity: human
application: flow cytometry

Flow cytometry analysis of human peripheral blood granulocytes stained with purified TRAIL-R3 (human), mAb (DJR3) (Prod. No. ALX-804-908), followed by biotinylated anti-mouse IgG and Sav-PE.
quantity: 100 µg
price:
to the supplier

TRAIL-R4 (human) monoclonal antibody (01)
Enzo Life Sciences
catalog: ALX-804-915-0100

mouse monoclonal (01)
reactivity: human
application: immunohistochemistry, flow cytometry

Immunohistochemistry staining of formalin fixed, paraffin-embedded human prostate tumor tissue using TRAIL-R2 (human), mAb (DJR2-2) at 1:50 dilution showing the positive staining in prostate carcinoma cells (63x).
quantity: 100 µg
price:
to the supplier