APRIL (human) monoclonal antibody (Aprily-8)
Enzo Life Sciences
catalog: ALX-804-149-C100

mouse monoclonal (Aprily-8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 2

Figure 3: Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min. at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50°C. Slides were then successively washed 2x 10 min. in xylol, 2x 10 min. in 100% ethanol, and then treated 10 min. in 100% methanol/0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% ethanol and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5µg/ml Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

Figure 2: FACS analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.
quantity: 100 µg
price:
to the supplier

APRIL (human) monoclonal antibody (Aprily-5)
Enzo Life Sciences
catalog: ALX-804-801-C100

mouse monoclonal (Aprily-5)
reactivity: human
application: western blot, ELISA, immunoprecipitation
citations: 2

Figure: Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5) (ALX-804-801). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2µg/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).
quantity: 100 µg
price:
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APRIL (human) monoclonal antibody (Aprily-5) (biotin conjugate)
Enzo Life Sciences
catalog: ALX-804-801B-C100

mouse monoclonal (Aprily-5)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
citations: 2

Figure: Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5) (ALX-804-801). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2µg/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).
quantity: 100 µg
price:
to the supplier

APRIL (human) monoclonal antibody (Aprily-2)
Enzo Life Sciences
catalog: ALX-804-844-C100

mouse monoclonal (Aprily-2)
reactivity: human
application: immunohistochemistry
citations: 3

Figure: Biopsy of tumor lesion from a patient with lymphoma (DLBCL) stained with MAb to APRIL (Aprily-2) (ALX-804-844). The biopsy was stained by standard immunohistochemistry. The figure shows the selective accumulation of APRIL around tumor cells.Picture courtesy of Dr. Bertrand Huard (University of Geneva).
quantity: 100 µg
price:
to the supplier

APRIL (human) monoclonal antibody (Aprily-1)
Enzo Life Sciences
catalog: ALX-804-148-C100

mouse monoclonal (Aprily-1)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry
citations: 1

Figure 3:FACS analysis of cells with MAb to hAPRIL (Aprily-1).Method: HEK 293  cells were mock transfected or transfected with an expression plasmid enabling surface expression of a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.

Figure 2: Detection of endogenous human APRIL with MAb to APRIL (Aprily-1) after IP with recombinant human BCMA:Fc.Method: Cell supernatants were first precleared with protein-A sepharose and then subjected to immunoprecipitation with rhBCMA:Fc (Prod. No. ALX-522-026) and protein-A sepharose. Beads were washed twice with PBS. Immunoprecipitated materials were eluted with 2.5mM glycine pH 2.5 for 10 min., neutralized with Tris buffer and run on an SDS-PAGE gel and analyzed with 2µg/ml Aprily-1 by Western blot. Note: In some migrations endogenous soluble APRIL resolved as a doublet around 20kDa (dendritic cells: DC). MegaAPRIL™, Soluble (human) (rec.) (Prod. No. ALX-522-035) served as a positive control.
quantity: 100 µg
price:
to the supplier

APRIL monoclonal antibody (Sacha-2)
Enzo Life Sciences
catalog: ALX-804-804-C100

rat monoclonal (Sacha-2)
reactivity: human, mouse
application: ELISA, immunocytochemistry, flow cytometry
citations: 1

quantity: 100 µg
price:
to the supplier

APRIL polyclonal antibody
Enzo Life Sciences
catalog: ADI-CSA-836-E

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

Immunohistochemistry analysis of APRIL in human prostate tissue with APRIL antibody at 10µg/ml.

Western blot analysis of APRIL expression in Jurkat cells with APRIL antibody at 1µg/ml (A), 2µg/ml (B), and 4µg/ml (C).
quantity: 100 µg
price:
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APRIL monoclonal antibody (Sacha-1)
Enzo Life Sciences
catalog: ALX-804-141-C100

rat monoclonal (Sacha-1)
reactivity: human, mouse
application: immunocytochemistry, flow cytometry

Figure 2: Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

Figure 1: MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141) detects membrane-bound human APRIL by FACS.Method: HEK 293T  cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141).  After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
quantity: 100 µg
price:
to the supplier