Caspase-9 monoclonal antibody (5B4)
Enzo Life Sciences
catalog: ADI-AAM-139-E
mouse monoclonal (5B4)
reactivity: human, mouse, rat
application: western blot
citations: 5

Western blot analysis of Caspase-9 fragments expression in apoptosis induced Jurkat cells by Fas mAb, probed with Caspase-9 mAb (5B4).
quantity: 100 µg
price:
to the supplier
Caspase-9 monoclonal antibody (10-1-87)
Enzo Life Sciences
catalog: ADI-905-686-100
mouse monoclonal (10-1-87)
reactivity: human
application: western blot
citations: 2

Western blot analysis of MW marker (1), Jurkat (2), and Jurkat + staurosporine (3) cells probed with Caspase 9 mAb (10-1-87).
quantity: 100 µg
price:
to the supplier
Caspase-9 polyclonal antibody (Bur 73)
Enzo Life Sciences
catalog: ALX-210-015-R050
domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 50 µl
price:
to the supplier
Caspase-9 monoclonal antibody (2-22)
Enzo Life Sciences
catalog: ADI-AAM-149-C
mouse monoclonal (2-22)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
citations: 1

IHC Analysis: Human uterus, vessels tissue stained with Caspase-9, mAb (2-22) at 10µg/ml.

quantity: 25 µg
price:
to the supplier
Caspase-9 polyclonal antibody (Bur 49)
Enzo Life Sciences
catalog: ALX-210-014-R050
domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 50 µl
price:
to the supplier
Caspase-9 (human) polyclonal antibody
Enzo Life Sciences
catalog: ALX-210-838-R100
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

Figure: (C) Immunoprecipitation of caspase-9.Method: Extracts of 1x107 Jurkat cells were subjected to immunoprecipitation with Protein G-sepharose in the presence of rabbit preimmune serum (PI control, lane 1) or the antiserum to caspase-9 (lane 2). Lane 3 demonstrates that the antiserum is able to completely deplete caspase-9 from cell lysates, as no caspase-9 was detected in the supernatants after precipititaion.

Figure: (B) Detection of caspase-9 processing during apoptosis. The antiserum detects procaspase-9 (46kDa) and the intermediate cleavage products of 37kDa and 35 kDa. No cross-reactivity with other caspases is observed.Method: Jurkat cells were treated with 2µM staurosporine (Prod. No. ALX-380-014). After 4 hours cell lysates were prepared and separated by SDS-Page (2x106 cells/lane) under reducing conditions. Proteins were immunoblotted with rabbit anti-caspase-9 (1:1'000). Following incubation with peroxidase-conjugated secondary antibodies caspase-9 processing was detected by enhanced chemoluminescent staining. The closed arrow indicates procaspase-9 (46kDa), which processed to the p35 and p37 fragments (open arrows).
quantity: 100 µl
price:
to the supplier
Caspase-9 polyclonal antibody
Enzo Life Sciences
catalog: ALX-210-815-C100
domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot

Figure 1: Western blot analysis of caspase 9 with rat kidney tissue lysate using Caspase-9, pAb (prod. No. ALX-210-815).
quantity: 100 µg
price:
to the supplier
Caspase-9 (active) polyclonal antibody
Enzo Life Sciences
catalog: ALX-210-816-C100
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunoprecipitation

Figure: Western blot analysis of Jurkat cell lysate. Lane 1: untreatedLane 2: treated with etoposide for 6 hours
quantity: 100 µg
price:
to the supplier
Caspase-9 (human) polyclonal antibody
Enzo Life Sciences
catalog: BML-SA321-0100
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity: 100 µl
price:
to the supplier