DNA Mass Spectroscopy versus ELISA analysis: The 5-hmC DNA ELISA Kit (prod. no. ADI-900-225) quantifies 5-hmC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. DNA samples include: brain, kidney, liver, thymus, human embryonic stem cells and two carcinoma samples.
quantity: 96 wells
price:
to the supplier
Detection of 5-hMC by qPCR. The €œno treatment€ DNA will establish the level (i.e., Cp value) that is representative of complete hydroxymethylation at the interrogation site. Conversely, the €œ- control€ (unglucosylated sample) will indicate a level of no hydroxymethylation at the site. The €œ+Glucosyltransferase (5-hmC)€ i.e glucosylated sample, Cp value should be somewhere between the two, i.e., €œno treatment€ and €œ-control€. This will depend on the extent of hydroxymethylation at the interrogation site. GSRE digested unglucoslyated DNA (i.e., €œ- control€) will always yield a signal albeit at a higher Cp value than the other samples.
Overall principle of the BioArray„¢ 5-hMC methylation kit. Treatment of DNA containing 5hmC with 5-hmC Glucosyltransferase specifically adds a glucose moiety yielding glucosyl-5-hydroxymethylcytosine. Following digestion with GSREs (will specifically cleave DNA having cytosine, 5-methylcytosine, or 5-hydroxymethylcytosine in the recognition sequence. Glucosyl-5-hydroxymethylcytosine in the DNA will remain uncleaved.
quantity: 50 Tests
price:
to the supplier