SUMO-1 (human), (recombinant) (His-tag)
Enzo Life Sciences
catalog: BML-UW9195-0500
citations: 6

SUMOylation assays for RANGAP1 target protein using SUMO-1, 2 and 3. SUMOylated proteins were detected by Western blotting using the appropriate BIOMOL SUMO antibodies. Results demonstrate the formation of SUMOylated target proteins of the expected size in all ATP containing reactions (+). The absence of such conjugates in negative control reactions (€“) demonstrates that their formation is ATP dependent (required for E1 activation) and hence derived from the SUMO cascade.

SDS-PAGE Analysis of SUMO-1 protein. Lane 1: MW markers (top to bottom) 66, 45, 36, 29, 24, 20 and 16. Lane 2: 10ug SUMO-1. Protein runs at ~20kDa.
quantity: 500 µg
price:
to the supplier
SUMO-1 (human) (1-101), (recombinant)
Enzo Life Sciences
catalog: ALX-201-044-C250
citations: 3
quantity: 250 µg
price:
to the supplier
SUMO-1 (human) (1-97), (recombinant)
Enzo Life Sciences
catalog: ALX-201-045-C500
citations: 3
quantity: 500 µg
price:
to the supplier
Blocking peptide for SUMO-1 (human) (NT) pAb (Prod. No. BML-PW8330)
Enzo Life Sciences
catalog: BML-PP9470-0100

quantity: 100 µg
price:
to the supplier
Blocking peptide for SUMO-1 (human) (CT) pAb (Prod. No. BML-PW9460)
Enzo Life Sciences
catalog: BML-PP9475-0100

quantity: 100 µg
price:
to the supplier
SUMO-1-AMC
Enzo Life Sciences
catalog: BML-UW0040-0025


DeSUMOylation Assay: 1µM SENP1 CD, His6-tagged (Prod. No. BML-UW9760); 500nM SUMO-1-AMC (Prod. No. BML-UW0040); 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT.
quantity: 25 µg
price:
to the supplier
SUMO-1 (human), (recombinant) (agarose immobilized)
Enzo Life Sciences
catalog: BML-UW0095-0500

quantity: 0.5 ml
price:
to the supplier
[E93R]SUMO-1 (human), (recombinant) (GST-tag)
Enzo Life Sciences
catalog: BML-UW0175-0100

quantity: 100 µg
price:
to the supplier
SUMO-1 (93-97)-AMCA
Enzo Life Sciences
catalog: BML-UW0500-1000


HPLC trace

DeSUMOylation Assay: 2µM SENP1 (Prod. No. BML-UW9760); 100µM SUMO1-AMCA (Prod. No. BML-UW0500); 50mM TRIS, pH 8.0, 20mM NaCl, 5mM DTT.
quantity: 1 mg
price:
to the supplier
SUMO-1 (human), (recombinant) (biotin conjugate)
Enzo Life Sciences
catalog: BML-UW0545-0100


Western blot of biotinylated-SUMO-1 (BML-UW0545) SUMOylation assay. Bt-SUMO-1 conjugated to RanGAP1 (BML-UW9755) in the presence of SUMO E1 (BML-UW9330) and Ubc9 (BML-UW9320). Bt-SUMO-1 conjugated species detected with streptavidin-HRP.
quantity: 100 µg
price:
to the supplier
pro-SUMO-1 (human), (recombinant) (His-tag)
Enzo Life Sciences
catalog: BML-UW9190-0500

quantity: 500 µg
price:
to the supplier