domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
reactivity: human
application: immunohistochemistry
mouse monoclonal (53)
reactivity: human
application: western blot, ELISA, immunohistochemistry
citations: 1
reactivity: human
application: western blot, ELISA, immunohistochemistry
citations: 1
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2µg/ml anti-OPN 53.
Western blot analysis: Protein samples (MDA-MB-435 cell lysates, conditioned medium from MDA-MB-435 cells containing secreted OPN or recombinant protein) were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS-T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5µg/ml) overnight at 4°C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (87-B)
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
