Ubiquitin (human), (fluorescein labeled)
Enzo Life Sciences
catalog: BML-UW1240-0100
citations: 4
quantity: 100 µg
price:
to the supplier
Ubiquitin
Enzo Life Sciences
catalog: BML-UW8795-0005
citations: 7
quantity: 5 mg
price:
to the supplier
Ubiquitin-AMC
Enzo Life Sciences
catalog: BML-SE211-0025
citations: 2

Figure: DUB Assay: 50nM UCLH1, His6-tagged (UW9740); 500nM Ubiquitin-AMC; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT.

Figure:  HPLC analysis. Comparison of Prod. No. BML-SE-211Prod. No. BML-SE-211) (A) with contaminated alternatively sourced material (B).
quantity: 25 µg
price:
to the supplier
Ubiquitin aldehyde, (recombinant)
Enzo Life Sciences
catalog: BML-UW8450-0050
citations: 4

Figure 2: Typical DUB Assay.Method: 12.5nM UCH-L1 (PGP9.5) (human) (recombinant) (His) (Prod. No. BML-UW9740); 500nM Ubiquitin-AMC; 500nM Ubiquitin Aldehyde (recombinant); 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT.

Figure 1: Typical HPLC analysis of Ubiquitin Aldehyde (recombinant) (Prod. No. BML-UW8450). Method: Column: VYDAC 218TP54; A: 0.1% TFA/H2O; B: 0.1% TFA/CH3CN; Gradient: 25-45% B/20 mins. Monitoring: 230nm.
quantity: 50 µg
price:
to the supplier
Ubiquitin vinyl sulfone, (HA-tag)
Enzo Life Sciences
catalog: BML-UW0155-0025
citations: 1

Image: DUB active site probe assay: Western blot showing reactions containing HAUbVS only (lane 1), HAUbVS + GST-UCHL1 (lane 2, Prod. No. BML-UW9305), and HA-UbVS + USP2cd (lane 3, Prod. No. BML-UW9850). HAUbVS modified proteins detected using HA polyclonal antibody (Sigma - H6908) at 1:1000 dilution.
quantity: 25 µg
price:
to the supplier
Poly-ubiquitin chains (Ub2-16) (K48-linked)
Enzo Life Sciences
catalog: BML-UW0670-0100
citations: 1

Western blot of K48-linked polyubiquitin chains (Ub2-16), detected by ubiquitin polyclonal antibody (Prod. No. BML-UG9510 (1:1000)).
quantity: 100 µg
price:
to the supplier
Tri-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0780-0100
citations: 1

Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Ubiquitin (human), (recombinant) (GST-tag)
Enzo Life Sciences
catalog: BML-UW8620-0001
citations: 1
quantity: 1 mg
price:
to the supplier
[KallR]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0205-1000

quantity: 1 mg
price:
to the supplier
K6-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0210-0001

quantity: 1 mg
price:
to the supplier
K11-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0215-0001

quantity: 1 mg
price:
to the supplier
K27-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0220-0001

quantity: 1 mg
price:
to the supplier
K29-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0225-0001

quantity: 1 mg
price:
to the supplier
K33-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0230-0001

quantity: 1 mg
price:
to the supplier
K48-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0235-0001

quantity: 1 mg
price:
to the supplier
K63-only Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0240-0001

quantity: 1 mg
price:
to the supplier
[K6R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0245-0001

quantity: 1 mg
price:
to the supplier
[K11R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0250-0001

quantity: 1 mg
price:
to the supplier
[K27R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0255-0001

quantity: 1 mg
price:
to the supplier
[K29R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0260-0001

quantity: 1 mg
price:
to the supplier
[K33R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0265-0001

quantity: 1 mg
price:
to the supplier
[K63R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0275-0001

quantity: 1 mg
price:
to the supplier
[D77]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW0345-0001

quantity: 1 mg
price:
to the supplier
Di-ubiquitin (K63-linked)
Enzo Life Sciences
catalog: BML-UW0730-0050


SDS-PAGE of di-ubiquitin Ub2, K63-linked (Lane 1: Prod. No. BML-UW0730, 5µg), in comparison with Ub3, K63-linked (Lane 2: Prod. No. BML-UW0745, 5µg) and Ub4, K63-linked (Lane 3: Prod. No. BML-UW0715, 5µg).
quantity: 50 µg
price:
to the supplier
Tri-ubiquitin (K63-linked)
Enzo Life Sciences
catalog: BML-UW0745-0050


SDS-PAGE of tri-ubiquitin Ub3, K63-linked (Lane 2: Prod. No. BML-UW0745, 5µg), in comparison with Ub2, K63-linked (Lane 1: Prod. No. BML-UW0730, 5µg) and Ub4, K63-linked (Lane 3: Prod. No. BML-UW0715, 5µg).
quantity: 50 µg
price:
to the supplier
Di-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0775-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Tetra-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0785-0100


SDS-PAGE Analysis: Lane 1: MW marker, Lane 2: 1.0 ug of purified Tetra-ubiquitin (Ub4) (Prod. No. BML-UW0785).

Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Penta-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0790-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Hexa-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0795-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Hepta-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0800-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Octa-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0805-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Nona-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0810-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Deca-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0815-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Undeca-ubiquitin (linear)
Enzo Life Sciences
catalog: BML-UW0820-0100


Figure 1: SDS-PAGE comparison of linear polyubiquitin chains (all 2 µg/lane): Lane 1: Ub2 (Prod. No. BML-UW0775) Lane 2: Ub3 (Prod. No. BML-UW0780) Lane 3: Ub4 (Prod. No. BML-UW0785) Lane 4: Ub5 (Prod. No. BML-UW0790) Lane 5: Ub6 (Prod. No. BML-UW0795) Lane 6: Ub7 (Prod. No. BML-UW0800) Lane 7: Ub8 (Prod. No. BML-UW0805) Lane 8: Ub9 (Prod. No. BML-UW0810)Lane 9: Ub10 (Prod. No. BML-UW0815) Lane 10: Ub11 (Prod. No. BML-UW0820)
quantity: 100 µg
price:
to the supplier
Ubiquitin vinyl methyl ester, (HA-tag)
Enzo Life Sciences
catalog: BML-UW0880-0025


Figure: DUB active site probe assay: Western blot showing reactions containing HA-Ub-VME only (lane 1), HA-Ub-VME + GST-UCHL1 (lane 2, Prod. No. BML-UW9305), and HA-Ub-VME + USP2cd (lane 3, Prod. No. BML-UW9850). HA-Ub-VME modified proteins detected using HA-reactive polyclonal antibody (Sigma - H6908) at 1:1000 dilution.Typical assay set-up: HA-Ub-VME probe concentration: 0.5-5.0µM. Purified enzyme concentration: 0.1-1.0µM; Buffer: 50mM TRIS, pH7.5, 150mM NaCl, 1mM DTT. Incubate at 37°C for 30 minutes. Modification of DUB enzymes by site-directed HA-Ub-VME probes detected by Western blotting with either HA-reactive antibody (general reactivity) or an antibody specific to particular DUB of interest. Note: When probing complex solutions such as cell lysates the optimal amount of total sample protein required will depend on the specific lysate to be analyzed and must therefore be determined for the individual lysate of interest.
quantity: 25 µg
price:
to the supplier
[(Nε-biotinyl)Lys6]Ubiquitin
Enzo Life Sciences
catalog: BML-UW8470-0100

quantity: 100 µg
price:
to the supplier
[(Nε-biotinyl)Lys6, (Nε-biotinyl)Lys48]Ubiquitin
Enzo Life Sciences
catalog: BML-UW8475-0100

quantity: 100 µg
price:
to the supplier
[(Nε-biotinyl)Lys6, (Nε-biotinyl)Lys63]Ubiquitin
Enzo Life Sciences
catalog: BML-UW8480-0100

quantity: 100 µg
price:
to the supplier
Ubiquitin (human), (recombinant) (His-tag)
Enzo Life Sciences
catalog: BML-UW8610-0001

quantity: 1 mg
price:
to the supplier
[K48R]Ubiquitin (human), (recombinant) (untagged)
Enzo Life Sciences
catalog: BML-UW8615-0001

quantity: 1 mg
price:
to the supplier
Ubiquitin, (agarose immobilized)
Enzo Life Sciences
catalog: BML-UW8630-0500

quantity: 0.5 ml
price:
to the supplier
Tetra-ubiquitin (K48-linked)
Enzo Life Sciences
catalog: BML-UW8645-0025


SDS-PAGE Analysis. Lane 1: MW Marker, Lane 2: 1 µg Tetra-Ubiquitin
quantity: 25 µg
price:
to the supplier
Ubiquitin, (biotinylated)
Enzo Life Sciences
catalog: BML-UW8705-0100

quantity: 100 µg
price:
to the supplier
Ubiquitin+1, (recombinant) (His-tag)
Enzo Life Sciences
catalog: BML-UW8790-0100

quantity: 100 µg
price:
to the supplier
Ubiquitin5+1 (recombinant) (His-tag)
Enzo Life Sciences
catalog: BML-UW8855-0025

quantity: 25 µg
price:
to the supplier
Poly-ubiquitin chains (Ub2-7) (K63-linked)
Enzo Life Sciences
catalog: BML-UW9570-0100

quantity: 100 µg
price:
to the supplier
Di-ubiquitin (K48-linked)
Enzo Life Sciences
catalog: BML-UW9800-0100


quantity: 100 µg
price:
to the supplier
Ubiquitin (human), (recombinant)
Enzo Life Sciences
catalog: BML-UW0280-0001

quantity: 1 mg
price:
to the supplier