Enzo Life Sciences LAG3 antibody

LAG-3 (human) monoclonal antibody (17B4) (preservative free)
Enzo Life Sciences
catalog: ALX-804-806PF-C100

mouse monoclonal (17B4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
citations: 20

quantity: 100 µg
price:
to the supplier

LAG-3 (human) monoclonal antibody (17B4)
Enzo Life Sciences
catalog: ALX-804-806-C100

Western blot analysis of LAG-3 mAb (17B4) (Prod. No. ALX-804-806): Lane 1: MW marker, Lane 2: LAG-3 (human):Fc (human), (recombinant) (Prod. No. ALX-522-078).
quantity: 100 µg
price:
to the supplier

LAG-3 (human) monoclonal antibody (17B4) (FITC conjugate)
Enzo Life Sciences
catalog: ALX-804-806F-C100

mouse monoclonal (17B4)
reactivity: human
conjugate: FITC
application: flow cytometry
citations: 17

Figure: Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using LAG-3 (human), mAb (17B4) (Prod. No. ALX-804-806)). Method: Freshly dissociated single cell suspensions of renal cell carcinoma TILs are incubated with LAG-3 (human), mAb (17B4) (FITC) (Prod. No. ALX-804-806) (5µg/ml) and anti-MHC Class II molecules (PE) for 30 min. and washed twice in saline buffer. Additional staining with anti-CD3 allowed a gate analysis of total T cells. The LAG-3 and MHC II profiles of CD3+-gated cells for 3 patients are shown [5].

Figure: Specific inhibition of 17B4 staining.Method: LAG-3 (human), mAb (17B4) (FITC) (Prod. No. ALX-804-806F) (10µg/ml) (is preincubated with a specific peptide epitope (208b) or a control tetanus toxoid (TT) peptide at different molarities prior to staining of TILs. Stained cells are then analyzed by FACS.
quantity: 100 µg
price:
to the supplier