Apaf-1 (human) monoclonal antibody (2E12)
Enzo Life Sciences
catalog: ALX-804-348-C100
rat monoclonal (2E12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 7

Figures: Species cross-reactivity of MAb to Apaf-1 (clone 2E12) (ALX-804-348) by Western blot.A: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3) were probed with clone 2E12.B: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2) were probed with clone 2E12. Results: 2E12 is human specific and does not cross-react with mouse or rat Apaf-1.

Figure: Western blot analysis with anti-Apaf-1 (human) MAb (clone 2E12) revealing endogenous Apaf-1. Lysates from 106 HeLa, MCF-7, 293T, COS, Jurkat, and activated primary human T cells were probed with clone 2E12. COS (monkey) cells were used as a negative control.
quantity: 100 µg
price:
to the supplier
Apaf-1 polyclonal antibody
Enzo Life Sciences
catalog: ADI-AAP-300-E
domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1µg/ml in the (A) absence and (B) presence of blocking peptide.

Immunohistochemistry analysis of Apaf1 in human heart tissue with Apaf1 antibody at 1µg/ml.
quantity: 100 µg
price:
to the supplier
Apaf-1 monoclonal antibody (18H2)
Enzo Life Sciences
catalog: ALX-804-350-C100
rat monoclonal (18H2)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 3

Figure: Species cross-reactivity of MAbs to APAF-1 by WB. left: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3). right: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2).

Figures: Species cross-reactivity of MAb to Apaf-1 (clone 18H2) (ALX-804-350) by Western blot.A: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3) were probed with clone 18H2.B: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2) were probed with clone 18H2. Results: 18H2 is human and mouse specific but does not recognize rat Apaf-1.
quantity: 100 µg
price:
to the supplier
Apaf-1 (CT) polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-179-100
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1µg/ml dilution in the absence (A) or presence (B) of blocking peptide.

Immunohistochemistry analysis of Apaf1 in human skin tissue with Apaf1 antibody at 20µg/ml.
quantity: 100 µg
price:
to the supplier