Histone H3 (acetyl-Lys9) polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-705-100

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
modification: acetylated
application: western blot
citations: 1

Western blot analysis of Histone H3 (Acetyl-Lys9) using 3T3 cell extracts treated with TSA. Lane 1: Molecular Weight Markers, Lane 2: 3T3, Lane 3: 3T3 + TSA.
quantity: 100 µg
price:
to the supplier

[pSer28]Histone H3 polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-752-100

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
modification: phosphorylated
application: western blot

Western blot analysis of Histone H3 (pSer28) using HeLa cell extracts treated with Nocodazole. Lane 1: molecular weight markers, Lane 2: HeLa, Lane 3: HeLa + Nocodazole.
quantity: 100 µg
price:
to the supplier

Histone H3 (dimethyl-Lys9) polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-778-100

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
modification: methylated
application: western blot

Western blot analysis of MW marker (1), 3T3 (2), and K- 562 (3) cell lysates probed with Histone H3 (dimethyl-Lys9) pAb.
quantity: 100 µg
price:
to the supplier

[pSer10]Histone H3 polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-780-100

domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot

Western blot analysis of MW marker (1), untreated Jurkat (2), and calyculin A-treated Jurkat (3) cell lysates probed with Histone H3 (pSer10) pAb.
quantity: 100 µg
price:
to the supplier

[pSer28]Histone H3 polyclonal antibody
Enzo Life Sciences
catalog: ADI-KAP-CC012-D

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Western blot analysis of M-phase arrested HeLa using Histone H3 (pSer28) pAb

Immunocytochemistry analysis of Histone H3 (pSer28) on acetone fixed Hep-2.
quantity: 50 µl
price:
to the supplier

[K9-trimethyl]Histone H3 monoclonal antibody (6F12-H4)
Enzo Life Sciences
catalog: ALX-804-673-C050

mouse monoclonal (6F12-H4)
reactivity: human, mouse
modification: methylated
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

Figure 5: Peptide competition assay using MAb to Histone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673).Method: To determine the specificity of MAb to Histone H3 (K9 methylated) (6F12-H4), the antibody was diluted 1:200 in 0.5% NFDM in PBS-T and incubated for 6 hours at 4°C with a molar excess of peptides corresponding to aa 1-20 of human histone H3, containing either unmodified K9 (lanes 2-6), dimethylated K9 (lanes 8-12), or tri-methylated K9 (lanes 14-18). In each case, the molar excess of peptide over antibody was 100 fold, 50 fold, 20 fold, 10 fold, and 5 fold, respectively, as indicated by the arrowheads (from left to right) above the blot. The membrane containing histones prepared from HeLa cells was then incubated o/n at 4°C with the antibody/peptide solutions, and processed for Western blotting. As control, one aliquot of antibody was incubated with water only (lane 1). Lanes 7 and 13 were left empty.

Figure 4: Dot-blot analysis using MAb to Histone H3 (K9 methylated) (6F12-H4) (Prod. No. ALX-804-673).Figure courtesy of Susanne Opravil and Thomas Jenuwein, Institute of Molecular Pathology (IMP) Vienna, Austria.
quantity: 50 µg
price:
to the supplier