mouse monoclonal (C-2-10)
reactivity: human, mouse, rat, bovine, rhesus macaque
application: western blot, ELISA, immunocytochemistry
citations: 27
reactivity: human, mouse, rat, bovine, rhesus macaque
application: western blot, ELISA, immunocytochemistry
citations: 27


Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101) and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The nitrocellulose membrane was probed with purified clone C-2-10 mouse monoclonal anti-PARP (Prod. No. BML-SA249) at 1 µg/ml. Secondary antibody was GAM-AP (1/2000) and the nitrocellulose membrane was developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
quantity: 50 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, bovine
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 3
reactivity: human, mouse, bovine
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 3


Figure: Western blot using rabbit polyclonal anti PARP-1.Lane 1: recombinant human PARP-1 (ALX-201-053, 5 ng).Lane 2: Total HeLa cell extract.Lane 3: Total MEF PARP1+/+ cell extract.Lane 4: Total MEF PARP1-/- cell extract.Lane 5: Lysate (50 ug) from HeLa cells.Lane 6: Lysate (50 ug) from HeLa PARP-1sh cells.Lane 7: Lysate (50 ug) from HeLa cells treated for 8 hours with Doxorubicin 5 ug/ml.Lane 8: Lysate (50 ug) from HEK293 cells.Lane 9: Lysate (50 ug) from HEK293 cells.Lane 10: Lysate (50 ug) from HEK293 cells transfected with PARP-1 siRNA.Lane 11: Lysate (50 ug) from HEK293 cells transfected with control siRNA.
quantity: 100 µl
price:
to the supplier
mouse monoclonal (F1-23)
reactivity: human, bovine
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 11
reactivity: human, bovine
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 11
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, ELISA
citations: 1
reactivity: human, mouse, rat, bovine
application: western blot, ELISA
citations: 1
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, ELISA
citations: 1
reactivity: human, mouse, rat, bovine
application: western blot, ELISA
citations: 1