BAFF monoclonal antibody (Buffy-2)
Enzo Life Sciences
catalog: ALX-804-131-C100

rat monoclonal (Buffy-2)
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 13

Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2.Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.
quantity: 100 µg
price:
to the supplier

BAFF monoclonal antibody (Buffy-2) (FITC conjugate)
Enzo Life Sciences
catalog: ALX-804-131F-T100

rat monoclonal (Buffy-2)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
citations: 13

Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.

Flow cytometry analysis of 106 Jurkat cells were stained using BAFF, mAb (Buffy-2) fluorescein conjugate at a concentration of 50µg/ml.
quantity: 100 tests
price:
to the supplier

BAFF (CT) polyclonal antibody
Enzo Life Sciences
catalog: ALX-210-799-C100

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry

Western blot analysis of BAFF in human HL60 cell lysate (A) and murine spleen tissue lysate (B) with PAb to BAFF (CT) (Prod. No. ALX-210-799) at 1 µg/ml.
quantity: 100 µg
price:
to the supplier

BAFF (mouse) monoclonal antibody (1C9)
Enzo Life Sciences
catalog: ALX-804-157-C100

rat monoclonal (1C9)
reactivity: mouse
application: ELISA, flow cytometry
citations: 1

Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (1C9). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
quantity: 100 µg
price:
to the supplier

BAFF (mouse) monoclonal antibody (1C9) (biotin conjugate)
Enzo Life Sciences
catalog: ALX-804-157B-C100

rat monoclonal (1C9)
reactivity: mouse
conjugate: biotin
application: ELISA, flow cytometry
citations: 1

quantity: 100 µg
price:
to the supplier

BAFF (mouse) monoclonal antibody (5A8)
Enzo Life Sciences
catalog: ALX-804-158-C100

rat monoclonal (5A8)
reactivity: mouse
application: western blot, ELISA, flow cytometry
citations: 2

Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
quantity: 100 µg
price:
to the supplier