rat monoclonal (3C5)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, other, western blot knockout validation
citations: 13
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, other, western blot knockout validation
citations: 13
Figure A: Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.Figure B: Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) recognizes all known isoforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), which detected EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 MAb was used as a loading control for all blots.Figure C: Anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) by Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) and detection by ECL. Probing with an anti-Hsp-70 MAb was used as a loading control.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (3E8)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, other
citations: 4
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, other
citations: 4
