BAFF monoclonal antibody (Buffy-2)
Enzo Life Sciences
catalog: ALX-804-131-C100

rat monoclonal (Buffy-2)
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 13

Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2.Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.
quantity: 100 µg
price:
to the supplier

BAFF monoclonal antibody (Buffy-2) (FITC conjugate)
Enzo Life Sciences
catalog: ALX-804-131F-T100

rat monoclonal (Buffy-2)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
citations: 13

Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.

Flow cytometry analysis of 106 Jurkat cells were stained using BAFF, mAb (Buffy-2) fluorescein conjugate at a concentration of 50µg/ml.
quantity: 100 tests
price:
to the supplier

BAFF (human) monoclonal antibody (Buffy-1)
Enzo Life Sciences
catalog: ALX-804-128-C100

rat monoclonal (Buffy-1)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
citations: 5

Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4µg/ml of MAb to BAFF (human) (Buffy-1). After washing in FACS buffer, FITC-conjugated antibody to rat IgG(ALX-211-051) was added and cells were analyzed by flow cytometry.

Membrane-bound human BAFF detected by MAb to BAFF (human) (Buffy-1) (Prod. No. ALX-804-128). Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control.
quantity: 100 µg
price:
to the supplier

BAFF (human) monoclonal antibody (Buffy-1) (FITC conjugate)
Enzo Life Sciences
catalog: ALX-804-128F-T100

rat monoclonal (Buffy-1)
reactivity: human
conjugate: FITC
application: western blot, immunocytochemistry, flow cytometry
citations: 5

Schematic structure of human soluble BAFF (rec.) (aa 83-285). Recognizes the stalk region present in the membrane-bound form of hBAFF.
quantity: 100 tests
price:
to the supplier

BAFF (CT) polyclonal antibody
Enzo Life Sciences
catalog: ALX-210-799-C100

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry

Western blot analysis of BAFF in human HL60 cell lysate (A) and murine spleen tissue lysate (B) with PAb to BAFF (CT) (Prod. No. ALX-210-799) at 1 µg/ml.
quantity: 100 µg
price:
to the supplier

BAFF (human) monoclonal antibody (Buffy-XI)
Enzo Life Sciences
catalog: ALX-804-147-C100

mouse monoclonal (Buffy-XI)
reactivity: human
application: western blot

Figure: Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector.Method: 10µg of total protein lysate was applied to the gel. MAb to BAFF (Buffy-XI) was used at 1µg/ml and revealed with HRP-coupled anti-mouse secondary antibody (1:4'000).
quantity: 100 µg
price:
to the supplier

BAFF (human) monoclonal antibody (4D3)
Enzo Life Sciences
catalog: ALX-804-159-C100

mouse monoclonal (4D3)
reactivity: human
application: immunohistochemistry, flow cytometry

quantity: 100 µg
price:
to the supplier