ELISA/assay

Zika virus IgG ELISA Assay Kit

ZIV31-K01

1 x 96 well

615 USD

Zika virus IgG ELISA Assay Kit

This Eagle Biosciences“sandwich” ELISA is designed, developed and produced for the qualitative measurement of IgG antibodies in blood specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antigen onto the wall of microtiter wells. Controls and blood specimen are added to microtiter wells of microplate that is coated with a highly purified Zika antigen on its wall. During the assay, Zika antibodies will be bound to the antigen coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated polyclonal antibody which specifically recognizes human IgG is added for further immunoreactions. After an incubation period, the immunocomplex of “Zika viral antigen – anti Zika antibodies – HRP-conjugated goat anti human IgG Tracer Antibody” is formed if Zika IgG antibodies are present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Zika IgG antibodies captured on the wall of each microtiter well is directly proportional to the amount of Zika IgG antibody level in each test specimen.

ELISA

Assay Kits

Immunology

1 x 96 well

Zika IgM

Zika IgG

Cut-off

10 µl

Serum, Plasma

2 hours

Human

RUO

wet

2-8C

615 USD