ELISA/assay

Vasoactive Intestinal Polypeptide (VIP) ELISA Assay kit

VIP31-K01

1 x 96 wells

570 USD

Vasoactive Intestinal Polypeptide (VIP) ELISA Assay kit

The VIP ELISA is a two-step competitive immunoassay. In the first incubation step, competition occurs between VIP present in calibrators, controls, specimen samples and a biotin-labeled antigen (biotin conjugate) for a limited number of anti-VIP antibody binding sites on the microplate wells. Excess and unbound materials are removed by a washing step. In the second incubation step, streptavidin-HRP conjugate is added, which binds specifically to any bound biotin conjugate. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of VIP present. Following an incubation, the enzymatic reaction is terminated by the additional of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of VIP in specimen samples and controls can be directly read.

ELISA

Assay Kit

Gastrointestinal, Immunology

1 x 96 wells

Vasoactive Intestinal Peptide, VIP

Vasoactive Intestinal Peptide, VIP

0 - 800 pg/mL

75 uL

EDTA plasma

2 hours 30 minutes

human

RUO

wet

4°C

570 USD