25-OH Vitamin D ELISA | Eagle BioSciences VID31-K01 product information

product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

25-OH Vitamin D ELISA

catalog :

VID31-K01

quantity :

1 x 96 well

price :

545 USD

more info or order :

Eagle BioSciences product webpage

citations: 3

Reference
de la Croix Ndong J, Makowski A, Uppuganti S, Vignaux G, Ono K, Perrien D, et al. Asfotase-? improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat Med. 2014;20:904-10 pubmed publisher
Jain S, Micinski D, Huning L, Kahlon G, Bass P, Levine S. Vitamin D and L-cysteine levels correlate positively with GSH and negatively with insulin resistance levels in the blood of type 2 diabetic patients. Eur J Clin Nutr. 2014;68:1148-53 pubmed publisher
Fidan V, Alp H, Gözeler M, Karaaslan O, Binay O, Cingi C. Variance of melatonin and cortisol rhythm in patients with allergic rhinitis. Am J Otolaryngol. 2013;34:416-9 pubmed publisher

product information

Eagle Catalog Number :

VID31-K01

Description :

25-OH Vitamin D ELISA

Assay Principle :

The new 25-OH Vitamin D ELISA assay test kit is designed for the determination of 25-OH Vitamin D in human serum or plasma samples. In the first analysis step, the calibrators and patient samples are diluted with biotin-labelled 25-OH vitamin D and added to microplate wells coated with monoclonal anti-25-OH Vitamin D antibodies. During the incubation an unknown amount of 25-OH Vitamin D in the patient sample and a known amount of biotin-labelled 25-OH vitamin D compete for the antibody binding sites in the microplate wells plate. Unbound 25-OH vitamin D is removed by washing. For the detection of bound biotin-labelled 25-OH vitamin D, a second incubation is performed using peroxidase-labelled streptavidin. In a third incubation using the peroxidase substrate tetramethylbenzidine (TMB) the bound peroxidase promotes a colour reaction. The colour intensity is inversely proportional to the 25-OH vitamin D concentration.

Product Type :

ELISA

Product Category :

Assay Kits

Product Subcategory :

Bone Metabolism

Size :

1 x 96 well

Biomarker :

Vitamin D

Keywords :

Vitamin D, 25-OH, 25 hydroxy, Cholecalciferol, calcidiol, calcitriol

Sensitivity :

1.9 ng/mL

Standard Range :

4 - 120 ng/mL

Sample Size :

10 ul

Sample Type :

Serum, Plasma

Incubation Time :

2 hours 45 minutes

Species :

Human

Regulatory Status :

RUO

Shipping :

wet

Storage :

2-8C

LIST Price :

545 USD

more info or order :

Eagle BioSciences product webpage