ELISA/assay

Mouse / Rat 25-OH Vitamin D ELISA

VID21-K01

1 x 96 well

545 USD

Mouse / Rat 25-OH Vitamin D ELISA

Assay calibrators, controls and test samples are added directly to wells of a microtiter plate that is coated with specific anti-25-OH Vitamin D2, D3 antibody.  A buffer designed to release Vitamin D from binding proteins is then added to the wells. After the first incubation period, unbound material is washed away and biotinylated Vitamin D analogue is added to the wells and binds to remaining antibody sites. After the second incubation period, unbound biotin-D is washed away and horseradish peroxidase (HRP) conjugated streptavidin is added to each well. During the third incubation step, an immune complex of well coated “vitamin D antibody – vitamin D, biotin D and HRP conjugated streptavidin” is formed. The unbound matrix is removed in the subsequent washing steps. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is inversely proportional to the amount of total 25-OH Vitamin D2/3 in the test sample. A calibration curve is generated by plotting the absorbance versus the respective Vitamin D concentration for each calibrator on a 4-parameter or point to point curve fitting. The concentration of total 25-OH Vitamin D2/3 in test samples is determined directly from this calibration curve.

ELISA

Assay Kits

Bone Metabolism

1 x 96 well

Vitamin D

Vitamin D, 25-OH, 25 hydroxy, Cholecalciferol, calcidiol, calcitriol

0.95 ng/mL

9 - 150 ng/mL

25 µl

Serum, Plasma

2 hours

Mouse, Goat, Equine, Human, Chicken, Bovine

RUO

wet

2-8C

545 USD