Swine TNF-α ELISA KIT | Eagle BioSciences TNA51-K01 product information

product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

Swine TNF-α ELISA KIT

catalog :

TNA51-K01

quantity :

1 x 96 well

price :

530 USD

product information

Eagle Catalog Number :

TNA51-K01

Description :

Swine TNF-α ELISA KIT

Assay Background :

The Eagle Biosciences Swine Tumor Necrosis Factor Alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any TNF-α present is bound by the immobilized antibody and the detection antibody specific for TNF-α is binds to the combination of capture antibody- TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.

Assay Principle :

The Eagle Biosciences Swine Tumor Necrosis Factor Alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any TNF-α present is bound by the immobilized antibody and the detection antibody specific for TNF-α is binds to the combination of capture antibody- TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.

Product Type :

ELISA

Product Category :

Assay Kits

Product Subcategory :

Oncology

Size :

1 x 96 well

Biomarker :

TNF-Alpha

Keywords :

Tumor Necrosis Factor, TNF-α, TNF-Alpha

Sensitivity :

15 pg/ml

Standard Range :

31.25 - 1000 pg/ml

Sample Size :

100 µl

Sample Type :

Serum, Plasma, Cell Culture

Incubation Time :

3.0 hours

Species :

Swine

Regulatory Status :

RUO

Shipping :

wet

Storage :

2-8C

2025 List Price :

530 USD