ELISA/assay

Human TNF-a ELISA Assay Kit

TNA31-K01

1 x 96 well

665 USD

Human TNF-a ELISA Assay Kit

The Eagle Biosciences Human TNF Alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF-α is added to the wells and binds to the combination of capture antibody- TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.

ELISA

Assay Kits

Endocrinology

1 x 96 well

TNF-a

TNF-a

25 pg/ml

25-800 pg/ml

100 ul

Serum, Plasma, Cell culture supernatant

2.5 hours

Human

RUO

wet

2-8C

665 USD