ELISA/assay

Dog Thymidine Kinase (TK1) ELISA Kit

TK149-K01

1 x 96 well

665 USD

Dog Thymidine Kinase (TK1) ELISA Kit

In this assay the thymidine kinase (TK1) present in samples reacts with the anti-TK1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-TK1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TK1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TK1 in the test sample. The quantity of TK1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kits

Immunology

1 x 96 well

Thymidine Kinase

Thymidine Kinase, TK1

0.094 ng/ml

0.078 ng/ml - 5.0 ng/ml

100 µl

serum/plasma

2 hrs 30 mins

Canine

RUO

wet

2-8C

665 USD