ELISA/assay

Human TGF-β1 ELISA KIT

TB131-K01

1 x 96 well

530 USD

Human TGF-β1 ELISA KIT

The Eagle Biosciences Human TNF Beta 1 (TNF-β1) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human TNF Beta 1 (TNF-β1 ) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human TNF Beta 1 (TNF-β1) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

The Eagle Biosciences Human TNF Beta 1 (TNF-β1) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TGF-β1 is added to the wells and binds to the combination of capture antibody-TGF-β1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TGF-β1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TGF-β1 standard dilutions and TGF-β1 sample concentration determined.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

TGF-Beta

Transforming growth factor Beta 1, TGF Beta 1, TGF-Beta 1, TGF-β1, TGF-B1, TGFB1

15 pg/ml

62.5 - 1000 pg/ml

100 µl

Serum, Plasma, Cell Culture

3.5 hours

Human

RUO

wet

2-8C

530 USD