ELISA/assay

Corticosterone EIA Kit

SKT-205-96

1 x 96 well

465 USD

Corticosterone EIA Kit

This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

ELISA

Assay Kit

Oxidative Stress

1 x 96 well

Corticosterone

Corticosterone

18.6 pg/ml

78.125 - 10,000 pg/ml

50 μL

Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine

1.5 hours

Human

RUO

wet

2-8C

465 USD