ELISA/assay

Rabbit CRP ELISA

RRP71-K01

1 x 96 well

610 USD

Rabbit CRP ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay kit

Veterinary

1 x 96 well

CRP

CRP

1.610 ng/ml

6.25 ng/ml - 200 ng/ml

100 µl

Serum, plasma

25 minutes

Rabbit

RUO

wet

2-8C

610 USD