ELISA/assay

Human Retinol Binding Protein (RBP4) ELISA

RBP39-K01

1 x 96 well

515 USD

Human Retinol Binding Protein (RBP4) ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-RBP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Immunology

1 x 96 well

Retinol Binding Protein (RBP4) ELISA

Retinol Binding Protein (RBP4) ELISA

1.163 ng/ml

3.125 ng/ml - 100 ng/ml

100 µl

Serum, plasma, urine

80 minutes

Human

RUO

wet

2-8C

515 USD