ELISA/assay

Rat Alpha 1-Acid Glycoprotein ELISA

RAA29-K01

1 x 96 well

610 USD

Rat Alpha 1-Acid Glycoprotein ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1-Acid Glycoprotein present in samples reacts with the anti-Alpha 1-Acid Glycoprotein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AGP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AGP in the test sample. The quantity of AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay kit

Veterinary

1 x 96 well

Alpha 1-Acid Glycoprotein ELISA

Alpha 1-Acid Glycoprotein ELISA

3.245 ng/ml

12.5 - 400 ng/ml

100 µl

Biological Fluids

80 minutes

Rat

RUO

wet

2-8C

610 USD